Immunomagnetic nanoparticle based quantitative PCR for rapid detection of Salmonella

Padmavathy Bakthavathsalam, Vinoth Kumar Rajendran, Uttara Saran, Suvro Chatterjee, Baquir Mohammed Jaffar Ali

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

We have developed a rapid and sensitive method for immunomagnetic separation (IMS) of Salmonella along with their real time detection via PCR. Silica-coated magnetic nanoparticles were functionalized with carboxy groups to which anti-Salmonella antibody raised against heat-inactivated whole cells of Salmonella were covalently attached. The immuno-captured target cells were detected in beverages like milk and lemon juice by multiplex PCR and real time PCR with a detection limit of 104 cfu.mL-1 and 103 cfu.mL-1, respectively. We demonstrate that IMS can be used for selective concentration of target bacteria from beverages for subsequent use in PCR detection. PCR also enables differentiation of Salmonella typhi and Salmonella paratyphi A using a set of four specific primers. In addition, IMS-PCR can be used as a screening tool in the food and beverage industry for the detection of Salmonella within 3-4 h which compares favorably to the time of several days that is needed in case of conventional detection based on culture and biochemical methods.

Original languageEnglish
Pages (from-to)1241-1248
Number of pages8
JournalMicrochimica Acta
Volume180
Issue number13-14
DOIs
Publication statusPublished - Oct 2013
Externally publishedYes

Keywords

  • biofunctionalized nanoparticles
  • immunomagnetic separation
  • multiplex detection
  • PCR
  • Salmonella

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