Impedance monitoring of cell adhesion and growth on mesoporous membrane

Lianmei Jiang; Jianmiao Liu; Jian Shi; Xin Li; Hao Li; Jun Liu; Jiannong Ye; Yong Chen

doi:10.1016/j.mee.2010.12.019

Impedance monitoring of cell adhesion and growth on mesoporous membrane

Lianmei Jiang, Jianmiao Liu, Jian Shi, Xin Li, Hao Li, Jun Liu, Jiannong Ye, Yong Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We present a microdevice configuration for impedance monitoring of adherent cells cultured on the surface of mesoporous membranes. The device fabrication was based on a 3 layer PDMS process which allows easy integration of microelectrodes in close proximity to cultured cells. The measurements were performed with HeLa cells on 30 nm diameter membranes of polycarbonate and embedded Pt and Ag/AgCl electrodes. As expected, the impedance of the cell-membrane assembly changes rapidly during the first 30 min due to cell adhesion and it continuously increases until cell confluence at day 5. This membrane based device configuration should be useful for accurate and real-time monitoring in cell adhesion, growth and migration studies.

Original language	English
Pages (from-to)	1722-1725
Number of pages	4
Journal	Microelectronic Engineering
Volume	88
Issue number	8
DOIs	https://doi.org/10.1016/j.mee.2010.12.019
Publication status	Published - Aug 2011
Externally published	Yes

Keywords

Cell culture
Impedance spectroscopy
Mesoporous membrane

Access to Document

10.1016/j.mee.2010.12.019

Link to publication in Scopus

Fingerprint Dive into the research topics of 'Impedance monitoring of cell adhesion and growth on mesoporous membrane'. Together they form a unique fingerprint.

Cite this

@article{6b7fdb6d1e7d462bb4a40fc66fa97e6d,

title = "Impedance monitoring of cell adhesion and growth on mesoporous membrane",

abstract = "We present a microdevice configuration for impedance monitoring of adherent cells cultured on the surface of mesoporous membranes. The device fabrication was based on a 3 layer PDMS process which allows easy integration of microelectrodes in close proximity to cultured cells. The measurements were performed with HeLa cells on 30 nm diameter membranes of polycarbonate and embedded Pt and Ag/AgCl electrodes. As expected, the impedance of the cell-membrane assembly changes rapidly during the first 30 min due to cell adhesion and it continuously increases until cell confluence at day 5. This membrane based device configuration should be useful for accurate and real-time monitoring in cell adhesion, growth and migration studies.",

keywords = "Cell culture, Impedance spectroscopy, Mesoporous membrane",

author = "Lianmei Jiang and Jianmiao Liu and Jian Shi and Xin Li and Hao Li and Jun Liu and Jiannong Ye and Yong Chen",

year = "2011",

month = aug,

doi = "10.1016/j.mee.2010.12.019",

language = "English",

volume = "88",

pages = "1722--1725",

journal = "Microelectronic Engineering",

issn = "0167-9317",

publisher = "Elsevier",

number = "8",

}

TY - JOUR

T1 - Impedance monitoring of cell adhesion and growth on mesoporous membrane

AU - Jiang, Lianmei

AU - Liu, Jianmiao

AU - Shi, Jian

AU - Li, Xin

AU - Li, Hao

AU - Liu, Jun

AU - Ye, Jiannong

AU - Chen, Yong

PY - 2011/8

Y1 - 2011/8

N2 - We present a microdevice configuration for impedance monitoring of adherent cells cultured on the surface of mesoporous membranes. The device fabrication was based on a 3 layer PDMS process which allows easy integration of microelectrodes in close proximity to cultured cells. The measurements were performed with HeLa cells on 30 nm diameter membranes of polycarbonate and embedded Pt and Ag/AgCl electrodes. As expected, the impedance of the cell-membrane assembly changes rapidly during the first 30 min due to cell adhesion and it continuously increases until cell confluence at day 5. This membrane based device configuration should be useful for accurate and real-time monitoring in cell adhesion, growth and migration studies.

AB - We present a microdevice configuration for impedance monitoring of adherent cells cultured on the surface of mesoporous membranes. The device fabrication was based on a 3 layer PDMS process which allows easy integration of microelectrodes in close proximity to cultured cells. The measurements were performed with HeLa cells on 30 nm diameter membranes of polycarbonate and embedded Pt and Ag/AgCl electrodes. As expected, the impedance of the cell-membrane assembly changes rapidly during the first 30 min due to cell adhesion and it continuously increases until cell confluence at day 5. This membrane based device configuration should be useful for accurate and real-time monitoring in cell adhesion, growth and migration studies.

KW - Cell culture

KW - Impedance spectroscopy

KW - Mesoporous membrane

UR - http://www.scopus.com/inward/record.url?scp=79960035429&partnerID=8YFLogxK

U2 - 10.1016/j.mee.2010.12.019

DO - 10.1016/j.mee.2010.12.019

M3 - Article

AN - SCOPUS:79960035429

VL - 88

SP - 1722

EP - 1725

JO - Microelectronic Engineering

JF - Microelectronic Engineering

SN - 0167-9317

IS - 8

ER -