Impedance monitoring of cell adhesion and growth on mesoporous membrane

Lianmei Jiang; Jianmiao Liu; Jian Shi; Xin Li; Hao Li; Jun Liu; Jiannong Ye; Yong Chen

doi:10.1016/j.mee.2010.12.019

Impedance monitoring of cell adhesion and growth on mesoporous membrane

Lianmei Jiang, Jianmiao Liu, Jian Shi, Xin Li, Hao Li, Jun Liu, Jiannong Ye, Yong Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

We present a microdevice configuration for impedance monitoring of adherent cells cultured on the surface of mesoporous membranes. The device fabrication was based on a 3 layer PDMS process which allows easy integration of microelectrodes in close proximity to cultured cells. The measurements were performed with HeLa cells on 30 nm diameter membranes of polycarbonate and embedded Pt and Ag/AgCl electrodes. As expected, the impedance of the cell-membrane assembly changes rapidly during the first 30 min due to cell adhesion and it continuously increases until cell confluence at day 5. This membrane based device configuration should be useful for accurate and real-time monitoring in cell adhesion, growth and migration studies.

Original language	English
Pages (from-to)	1722-1725
Number of pages	4
Journal	Microelectronic Engineering
Volume	88
Issue number	8
DOIs	https://doi.org/10.1016/j.mee.2010.12.019
Publication status	Published - Aug 2011
Externally published	Yes

Keywords

Cell culture
Impedance spectroscopy
Mesoporous membrane

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10.1016/j.mee.2010.12.019

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Jiang, L., Liu, J., Shi, J., Li, X., Li, H., Liu, J., ... Chen, Y. (2011). Impedance monitoring of cell adhesion and growth on mesoporous membrane. Microelectronic Engineering, 88(8), 1722-1725. https://doi.org/10.1016/j.mee.2010.12.019

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AU - Jiang, Lianmei

AU - Liu, Jianmiao

AU - Shi, Jian

AU - Li, Xin

AU - Li, Hao

AU - Liu, Jun

AU - Ye, Jiannong

AU - Chen, Yong

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AB - We present a microdevice configuration for impedance monitoring of adherent cells cultured on the surface of mesoporous membranes. The device fabrication was based on a 3 layer PDMS process which allows easy integration of microelectrodes in close proximity to cultured cells. The measurements were performed with HeLa cells on 30 nm diameter membranes of polycarbonate and embedded Pt and Ag/AgCl electrodes. As expected, the impedance of the cell-membrane assembly changes rapidly during the first 30 min due to cell adhesion and it continuously increases until cell confluence at day 5. This membrane based device configuration should be useful for accurate and real-time monitoring in cell adhesion, growth and migration studies.

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