Improved detection limits of bacterial endotoxins using new type of planar interdigital sensors

A. R Mohd Syaifudin*, Asif I. Zia, S. C. Mukhopadhyay, P. L. Yu, Chinthaka P. Gooneratne, Jurgen Kosel

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

3 Citations (Scopus)

Abstract

New types of planar interdigital sensors were fabricated by photolithography and etching techniques on a Silicon/Silicon Dioxide (Si/SiO2) wafer (single side polished). The sensors were then coated with APTES (3-aminopropyltrietoxysilane) a cross linker used to bind Polymyxin B (PmB) molecules on electrodes surface. PmB is an antimicrobial peptide produced by the Gram-positive bacterium-Bacillus which has specific binding properties to Lipopolysaccharide (LPS). This paper will discuss the fabrication process, coating and immobilization procedures and analysis of sensors' performance based on Impedance Spectroscopy method. The sensor sensitivity was compared to standard ToxinSensor Chromogenic LAL Endotoxin Assay Kit for verification.

Original languageEnglish
Title of host publicationIEEE SENSORS 2012 - Proceedings
Place of PublicationPiscataway, NJ
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages1-4
Number of pages4
ISBN (Electronic)9781457717673
ISBN (Print)9781457717659
DOIs
Publication statusPublished - 2012
Externally publishedYes
Event11th IEEE SENSORS 2012 Conference - Taipei, Taiwan, Province of China
Duration: 28 Oct 201231 Oct 2012

Other

Other11th IEEE SENSORS 2012 Conference
CountryTaiwan, Province of China
CityTaipei
Period28/10/1231/10/12

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