The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus (Dictyostelium). Most particularly, the present invention relates to novel strains of the genus (Dictyostelium), recombinant plasmids for use with strains of the genus (Dictyostelium), and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus (Dictyostelium). In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in (Dictyostelium) spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type (Dictyostelium) spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.
|IPC||C12N 15/80,C12N 15/11,C12N 15/79|
|Publication status||Published - 27 Sep 1994|