Improved plasmid vectors for cellular slime moulds of the genus dictyostelium

Martin Basil Slade; Andy Chin Meng Chang; Keith Leslie Williams

Improved plasmid vectors for cellular slime moulds of the genus dictyostelium

Martin Basil Slade (Inventor), Andy Chin Meng Chang (Inventor), Keith Leslie Williams (Inventor)

Research output: Patent

Abstract

The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus Dictyostelium. Most particularly, the present invention relates to novel strains of the genus Dictyostelium, recombinant plasmids for use with strains of the genus Dictyostelium, and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus Dictyostelium. In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in Dictyostelium spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type Dictyostelium spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.

Original language	English
Patent number	WO1991006644
IPC	C07K 14/37 2006.1,C12N 15/80 2006.1
Priority date	2/11/89
Filing date	2/11/90
Publication status	Submitted - 16 May 1991

Bibliographical note

Designated States:
AT, AU, BB, BG, BR, CA, CH, DE, DK, ES, FI, GB, HU, JP, KP, KR, LK, LU, MG, MW, NO, RO, SD, SE, SU, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CM, GA, ML, MR, SN, TD, TG)

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2 Patent

Improved plasmid vectors for cellular slime moulds of the genus dictyostelium
Chang, A. C. M., Slade, M. B. & Williams, K. L., 27 Sept 1994, IPC No. C12N 15/80, C12N 15/11, C12N 15/79, Patent No. AU1990066249, Priority date 2 Nov 1990, Priority No. AUPJ7187
Research output: Patent

Open Access
Improved plasmid vectors for cellular slime moulds of the genus dictyostelium
Slade, M. B., Chang, A. C. M. & Williams, K. L., 19 Aug 1992, (Submitted) IPC No. C12N 15/79, C12N 15/11, C07K 14/37, C12N 15/80, C12N 1/15, C07K 14/00, C12N 15/80, Patent No. EP0498827, Priority date 2 Nov 1989, Priority No. AUPJ718789
Research output: Patent

Cite this

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title = "Improved plasmid vectors for cellular slime moulds of the genus dictyostelium",

abstract = "The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus Dictyostelium. Most particularly, the present invention relates to novel strains of the genus Dictyostelium, recombinant plasmids for use with strains of the genus Dictyostelium, and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus Dictyostelium. In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in Dictyostelium spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type Dictyostelium spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.",

author = "Slade, {Martin Basil} and Chang, {Andy Chin Meng} and Williams, {Keith Leslie}",

note = "Designated States: AT, AU, BB, BG, BR, CA, CH, DE, DK, ES, FI, GB, HU, JP, KP, KR, LK, LU, MG, MW, NO, RO, SD, SE, SU, US European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE) African Intellectual Property Organization (BF, BJ, CF, CG, CM, GA, ML, MR, SN, TD, TG); WO1991006644; C07K 14/37 2006.1,C12N 15/80 2006.1",

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TY - PAT

T1 - Improved plasmid vectors for cellular slime moulds of the genus dictyostelium

AU - Slade, Martin Basil

AU - Chang, Andy Chin Meng

AU - Williams, Keith Leslie

N1 - Designated States: AT, AU, BB, BG, BR, CA, CH, DE, DK, ES, FI, GB, HU, JP, KP, KR, LK, LU, MG, MW, NO, RO, SD, SE, SU, US European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE) African Intellectual Property Organization (BF, BJ, CF, CG, CM, GA, ML, MR, SN, TD, TG)

PY - 1991/5/16

Y1 - 1991/5/16

N2 - The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus Dictyostelium. Most particularly, the present invention relates to novel strains of the genus Dictyostelium, recombinant plasmids for use with strains of the genus Dictyostelium, and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus Dictyostelium. In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in Dictyostelium spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type Dictyostelium spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.

AB - The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus Dictyostelium. Most particularly, the present invention relates to novel strains of the genus Dictyostelium, recombinant plasmids for use with strains of the genus Dictyostelium, and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus Dictyostelium. In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in Dictyostelium spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type Dictyostelium spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.

M3 - Patent

M1 - WO1991006644

Y2 - 1990/11/02

ER -

Improved plasmid vectors for cellular slime moulds of the genus dictyostelium

Abstract

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