Improved plasmid vectors for cellular slime moulds of the genus dictyostelium

Martin Basil Slade (Inventor), Andy Chin Meng Chang (Inventor), Keith Leslie Williams (Inventor)

Research output: Patent

Abstract

The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus Dictyostelium. Most particularly, the present invention relates to novel strains of the genus Dictyostelium, recombinant plasmids for use with strains of the genus Dictyostelium, and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus Dictyostelium. In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in Dictyostelium spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type Dictyostelium spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.

Original languageEnglish
Patent numberWO1991006644
IPCC07K 14/37 2006.1,C12N 15/80 2006.1
Priority date2/11/89
Filing date2/11/90
Publication statusSubmitted - 16 May 1991

Bibliographical note

Designated States:
AT, AU, BB, BG, BR, CA, CH, DE, DK, ES, FI, GB, HU, JP, KP, KR, LK, LU, MG, MW, NO, RO, SD, SE, SU, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CM, GA, ML, MR, SN, TD, TG)

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