Improved plasmid vectors for cellular slime moulds of the genus dictyostelium

Martin Basil Slade; Andy Chin Meng Chang; Keith Leslie Williams

Improved plasmid vectors for cellular slime moulds of the genus dictyostelium

Martin Basil Slade (Inventor), Andy Chin Meng Chang (Inventor), Keith Leslie Williams (Inventor)

Research output: Patent

Abstract

The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus (Dictyostelium). Most particularly, the present invention relates to novel strains of the genus (Dictyostelium), recombinant plasmids for use with strains of the genus (Dictyostelium), and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus (Dictyostelium). In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in (Dictyostelium) spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type (Dictyostelium) spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.

Original language	English
Patent number	EP0498827
IPC	C12N 15/79,C12N 15/11,C07K 14/37,C12N 15/80,C12N 1/15,C07K 14/00,C12N 15/80
Priority date	2/11/89
Publication status	Submitted - 19 Aug 1992

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2 Patent

Improved plasmid vectors for cellular slime moulds of the genus dictyostelium
Chang, A. C. M., Slade, M. B. & Williams, K. L., 27 Sept 1994, IPC No. C12N 15/80, C12N 15/11, C12N 15/79, Patent No. AU1990066249, Priority date 2 Nov 1990, Priority No. AUPJ7187
Research output: Patent

Open Access
Improved plasmid vectors for cellular slime moulds of the genus dictyostelium
Slade, M. B., Chang, A. C. M. & Williams, K. L., 16 May 1991, (Submitted) IPC No. C07K 14/37 2006.1, C12N 15/80 2006.1, Patent No. WO1991006644, 2 Nov 1990, Priority date 2 Nov 1989, Priority No. AUPJ7187
Research output: Patent

Cite this

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title = "Improved plasmid vectors for cellular slime moulds of the genus dictyostelium",

abstract = "The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus (Dictyostelium). Most particularly, the present invention relates to novel strains of the genus (Dictyostelium), recombinant plasmids for use with strains of the genus (Dictyostelium), and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus (Dictyostelium). In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in (Dictyostelium) spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type (Dictyostelium) spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.",

author = "Slade, {Martin Basil} and Chang, {Andy Chin Meng} and Williams, {Keith Leslie}",

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T1 - Improved plasmid vectors for cellular slime moulds of the genus dictyostelium

AU - Slade, Martin Basil

AU - Chang, Andy Chin Meng

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N2 - The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus (Dictyostelium). Most particularly, the present invention relates to novel strains of the genus (Dictyostelium), recombinant plasmids for use with strains of the genus (Dictyostelium), and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus (Dictyostelium). In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in (Dictyostelium) spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type (Dictyostelium) spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.

AB - The present invention relates generally to the fields of molecular biology and the production of recombinant protein using cellular slime moulds of the genus (Dictyostelium). Most particularly, the present invention relates to novel strains of the genus (Dictyostelium), recombinant plasmids for use with strains of the genus (Dictyostelium), and polypeptides which facilitate the extrachromosomal replication of such plasmids in strains of the genus (Dictyostelium). In particular, the present invention provides a polypeptide which facilitates the extrachromosomal replication of a recombinant plasmid in (Dictyostelium) spp in which the recombinant plasmid includes an origin of replication derived from a Ddp2-like plasmid but which lacks functional genes for extrachromosomal replication in wild type (Dictyostelium) spp. The extrachromosomal replicating plasmid constructed in accordance with the present invention are suitable for carrying a wide variety of genes and promoter sequences for control production of recombinant proteins by the biotechnology industry.

M3 - Patent

M1 - EP0498827

ER -

Improved plasmid vectors for cellular slime moulds of the genus dictyostelium

Abstract

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