Improvements for the visualization of low-molecular weight protein and peptides of human tears using MALDI

Terry Nguyen-Khuong, Anna Fitzgerald, Zhejun Zhao, Mark Willcox, Bradley J. Walsh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Many low-molecular weight proteins and peptides in human tears are potentially bioactive proteins but the range and number of these is yet to be fully characterized. A number of different sample preparation techniques were used to maximize the visualization of peptides from reflex tears. Samples were pretreated using precipitation and filtration techniques prior to analyses using MALDI-TOF MS. Peptides were searched for between 700 to 4000 m/z. Sample dilution in several different buffer systems followed by filtration with a 30-kDa cutoff filter and C18 reverse phase microcolumn purification produced significantly (p = 0.049) more peaks in tears than other methods used to prepare tears prior to MALDI-TOF MS. This study has established a technique for optimizing the visualization of naturally occurring peptides in tears.

Original languageEnglish
Pages (from-to)3424-3432
Number of pages9
JournalProteomics
Volume8
Issue number17
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

    Fingerprint

Cite this