Improving single-target encapsulation efficiency using viscoelastic medium

Siyuan Zhuang; Ling Liu; Yoichiroh Hosokawa; Yalikun Yaxiaer; David Inglis; Ming Li

Improving single-target encapsulation efficiency using viscoelastic medium

Siyuan Zhuang, Ling Liu, Yoichiroh Hosokawa, Yalikun Yaxiaer, David Inglis, Ming Li^*

^*Corresponding author for this work

School of Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

This study demonstrates a passive controlled single-particle encapsulation using viscoelastic microfluidics. polyethylene oxide (PEO) drives particles focusing at a certain cross-sectional position in the channel. The proposed method enables particle focusing and overcomes the difficulties of forming uniform droplets due to the long filament thread of viscoelastic liquid at high flow rates. Therefore, the probability of single-particle encapsulation rate achieved up to 2-fold better than that predicted by Poisson statistics. We expect this approach to provide higher throughput for single-cell analysis with an adaption of flow cytometric assays.

Original language	English
Title of host publication	MicroTAS 2022
Subtitle of host publication	The 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of Publication	California
Publisher	Chemical and Biological Microsystems Society
Pages	807-808
Number of pages	2
ISBN (Electronic)	9781733419048
Publication status	Published - 2022
Event	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 - Hybrid, Hangzhou, China Duration: 23 Oct 2022 → 27 Oct 2022

Publication series

Name
ISSN (Electronic)	1556-5904

Conference

Conference	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Country/Territory	China
City	Hybrid, Hangzhou
Period	23/10/22 → 27/10/22

Keywords

particle ordering
single-cell encapsulation
double emulsions

Cite this

@inproceedings{d3e573dbb33f4237be9b8a0a547c399b,

title = "Improving single-target encapsulation efficiency using viscoelastic medium",

abstract = "This study demonstrates a passive controlled single-particle encapsulation using viscoelastic microfluidics. polyethylene oxide (PEO) drives particles focusing at a certain cross-sectional position in the channel. The proposed method enables particle focusing and overcomes the difficulties of forming uniform droplets due to the long filament thread of viscoelastic liquid at high flow rates. Therefore, the probability of single-particle encapsulation rate achieved up to 2-fold better than that predicted by Poisson statistics. We expect this approach to provide higher throughput for single-cell analysis with an adaption of flow cytometric assays.",

keywords = "particle ordering, single-cell encapsulation, double emulsions",

author = "Siyuan Zhuang and Ling Liu and Yoichiroh Hosokawa and Yalikun Yaxiaer and David Inglis and Ming Li",

year = "2022",

language = "English",

publisher = "Chemical and Biological Microsystems Society",

pages = "807--808",

booktitle = "MicroTAS 2022",

note = "26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 ; Conference date: 23-10-2022 Through 27-10-2022",

}

Zhuang, S, Liu, L, Hosokawa, Y, Yaxiaer, Y, Inglis, D & Li, M 2022, Improving single-target encapsulation efficiency using viscoelastic medium. in MicroTAS 2022: The 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, California, pp. 807-808, 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022, Hybrid, Hangzhou, China, 23/10/22.

Improving single-target encapsulation efficiency using viscoelastic medium. / Zhuang, Siyuan; Liu, Ling; Hosokawa, Yoichiroh et al.
MicroTAS 2022: The 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. California: Chemical and Biological Microsystems Society, 2022. p. 807-808.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Improving single-target encapsulation efficiency using viscoelastic medium

AU - Zhuang, Siyuan

AU - Liu, Ling

AU - Hosokawa, Yoichiroh

AU - Yaxiaer, Yalikun

AU - Inglis, David

AU - Li, Ming

PY - 2022

Y1 - 2022

N2 - This study demonstrates a passive controlled single-particle encapsulation using viscoelastic microfluidics. polyethylene oxide (PEO) drives particles focusing at a certain cross-sectional position in the channel. The proposed method enables particle focusing and overcomes the difficulties of forming uniform droplets due to the long filament thread of viscoelastic liquid at high flow rates. Therefore, the probability of single-particle encapsulation rate achieved up to 2-fold better than that predicted by Poisson statistics. We expect this approach to provide higher throughput for single-cell analysis with an adaption of flow cytometric assays.

AB - This study demonstrates a passive controlled single-particle encapsulation using viscoelastic microfluidics. polyethylene oxide (PEO) drives particles focusing at a certain cross-sectional position in the channel. The proposed method enables particle focusing and overcomes the difficulties of forming uniform droplets due to the long filament thread of viscoelastic liquid at high flow rates. Therefore, the probability of single-particle encapsulation rate achieved up to 2-fold better than that predicted by Poisson statistics. We expect this approach to provide higher throughput for single-cell analysis with an adaption of flow cytometric assays.

KW - particle ordering

KW - single-cell encapsulation

KW - double emulsions

UR - https://www.scopus.com/pages/publications/85175725988

UR - http://purl.org/au-research/grants/arc/DP200102269

UR - https://cbmsociety.org/conference/proceedings_archive.html

M3 - Conference proceeding contribution

AN - SCOPUS:85175725988

SP - 807

EP - 808

BT - MicroTAS 2022

PB - Chemical and Biological Microsystems Society

CY - California

T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022

Y2 - 23 October 2022 through 27 October 2022

ER -

Improving single-target encapsulation efficiency using viscoelastic medium

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Improving single-target encapsulation efficiency using viscoelastic medium

Abstract

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