In silico predicted mycobacterial epitope elicits in vitro T-cell responses

Md Kawsar Khan, Shabnam Zaman, Sajib Chakraborty, Rajib Chakravorty, Mohammad Murshid Alam, Taufiqur Rahman Bhuiyan, Muhammad Jubayer Rahman, Carmen Fernández, Firdausi Qadri, Zeba I. Seraj*

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Epitope-based vaccines permit the selection of only a specific subset of epitopes to induce the necessary immune response, thus providing a rational alternative to conventional design approaches. Using a range of immunoinformatics tools, we identified a novel, contiguous 28 amino acid multi-epitope cluster within the highly conserved secretory protein Ag85B of Mycobacterium tuberculosis, the causative agent of TB. This cluster, named Ep85B, is composed of epitopes which bind to three HLA Class I and 15 Class II molecules, and harbors the potential to generate 99% population coverage in TB-endemic regions. We experimentally evaluated the capacity of Ep85B to elicit T-cell immune responses using whole blood cells and, as predicted, observed significant increases in populations of both CD4+ and memory CD4+ CD45RO+ T-cells. Our results demonstrate the practical utility of an epitope-based design methodology - a strategy that, following further evaluation, may serve as an additional tool for the development of novel vaccine candidates against TB and other diseases.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalMolecular Immunology
Volume61
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Ag85B
  • Epitope vaccine
  • Immunoinformatics
  • Tuberculosis

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