In-situ recovery of persipeptides from Streptomyces zagrosensis fermentation broth by enhanced adsorption

Hamed Kazemi Shariat Panahi, Fatemeh Mohammadipanah*, Farzaneh Rahmati, Aliakbar Tarlani, Javad Hamedi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
1 Downloads (Pure)


Background: Drug discovery process is growing considerably due to the noteworthy resource of natural products. Persipeptides A and B are cyclopeptide antibiotics, which are produced by Streptomyces zagrosensis UTMC 1154. Although extraction of culture broth with the help of solvent has been optimized previously, no effort for in-situ extraction of persipeptides has been done yet. Objective: To produce a high quantity of persipeptides for further drug evaluation, it is crucial to design approaches aimed at improvement of the extraction yield. Materials and Methods: Amberlite XAD-16N was employed into the fermentation culture medium of S. zagrosensis in order to enhance the in-situ extraction of persipeptides. Effects of resin content (%), resin addition time (h), and fermentation time (day) were investigated by a two-level full factorial experimental design. Results: The main factors of resin content (%) and the interaction of resin content (%) with resin addition time (day) were found to be important using ANOVA. The results showed the amount of 0.33 % (w.v-1) amberlite XAD-16N added at 27.2 h post-inoculation was the most effective combination to increase the efficiency of in-situ adsorption capacity of persipeptides. Conclusions: The provided method requires 3.3 g resin and 200 mL methanol for the extraction of persipeptides from each liter of fermentation culture of S. zagrosensis in less than 15 min. Apart from cost-efficiently and simplicity, this procedure enhanced the recovery of persipeptides by 7 % and 3 times, compared to ISP2 medium without any resin after 4 and 7 days of fermentation, respectively. Therefore, this method can be regarded as a cost-efficient enhancement approach for the production of these newly-discovered metabolites before implementing the genetic manipulation or intensive media optimization, demanding considerable time and effort.

Original languageEnglish
Article numbere2231
Pages (from-to)1-10
Number of pages10
JournalIranian Journal of Biotechnology
Issue number2
Publication statusPublished - Apr 2020

Bibliographical note

Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.


  • Amberlite Resin
  • Experimental design
  • Full factorial optimization
  • In situ metabolic extraction
  • Natural product
  • Persipeptides


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