In vitro and in vivo biotinylation of endothelial cell surface proteins in the pursuit of targets for molecular therapies for brain AVMs

Identification of membrane proteins that are expressed on the endothelium after radiosurgery is of fundamental importance in developing a new treatment for brain vascular formations. We optimized then employed in vitro and in vivo biotinylation methodology to label membrane proteins in a murine cerebral endothelial cell cultures (bEnd.3) and a rat model of arteriovenous malformation (AVM). Membrane proteins were then captured on streptavidin resin and identified using proteomics analysis.