In vitro and in vivo imaging of xenobiotic transport in human skin and in the rat liver

Michael S. Roberts*, Matthew J. Roberts, Thomas A. Robertson, Washington Sanchez, Camilla Thörling, Yuhong Zou, Xin Zhao, Wolfgang Becker, Andrei V. Zvyagin

*Corresponding author for this work

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Multiphoton tomography was used to examine xenobiotic transport in vivo. We used the photochemical properties of zinc oxide and fluorescein and multiphoton tomography to study their transport in the skin and in the rat liver in vivo. Zinc oxide nanoparticles were visualised in human skin using the photoluminescence properties of zinc oxide and either a selective emission wavelength band pass filter or a filter with fluorescence lifetime imaging (FLIM). Zinc oxide nanoparticles (30 nm) did not penetrate into human skin in vitro and in vivo and this was validated by scanning electron microscopy with Xray photoelectron spectroscopy. Fluorescein was measured in the liver using FLIM. Fluorescein is rapidly extracted from the blood into the liver cells and then transported into the bile. It is suggested that multiphoton tomography may be of particular use in defining in vivo 4D (in both space and time) pharmacokinetics.

Original languageEnglish
Pages (from-to)478-493
Number of pages16
JournalJournal of Biophotonics
Volume1
Issue number6
DOIs
Publication statusPublished - 2008

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