In vitro effects of noradrenaline on Sydney rock oyster (Saccostrea glomerata) hemocytes

Saleem Aladaileh, Mohammad G. Mohammad, Belinda Ferrari, Sham V. Nair, David A. Raftos*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)


    Our prior work has shown that the catecholamine hormone, noradrenaline, mediates environmental stress responses in Sydney rock oysters, resulting in impaired immunological function. In the current study, we tested the cellular basis of this stress response. Hemocytes were exposed to noradrenaline in vitro before cell morphology and viability were analyzed. Noradrenaline was shown to induce apoptotic markers, including the loss of mitochondrial membrane potential, DNA fragmentation and plasma membrane blebbing. F-actin appeared to play an important role in the changes observed in hemocytes, being concentrated mostly in the plasma membrane blebs of noradrenaline-treated hemocytes. This may explain why hemocyte adhesion and pseudopodia formation were inhibited by noradrenaline. Cellular dysfunction induced by norarenaline mainly affected the hyalinocyte sub-population of hemocytes, whilst the other major cell type, granulocytes, remained unaffected. Given that hyalinocytes are important immunological effectors, the results of this study help to explain why immunosuppression accompanies noradrenaline-mediated stress responses in oysters.

    Original languageEnglish
    Pages (from-to)691-697
    Number of pages7
    JournalComparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology
    Issue number4
    Publication statusPublished - Dec 2008


    Dive into the research topics of 'In vitro effects of noradrenaline on Sydney rock oyster (Saccostrea glomerata) hemocytes'. Together they form a unique fingerprint.

    Cite this