In vitro mutagenesis of a xylanase from the extreme thermophile Caldocellum saccharolyticum

Ernst Lüthi*, Karin Reif, Nila Bhana Jasmat, Peter L. Bergquist

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Six mutant xylanases were obtained by in vitro mutagenesis of a xylanase gene from the extremely thermophilic bacterium Caldocellum saccharolyticum. The temperature stability of all enzymes was affected by mutation to various degrees and one of the xylanases had an altered temperature optimum. The mutations had no effect on the pH optimum. The C. saccharolyticum xylanase showed strong homology to several thermophilic and mesophilic xylanases, and comparison of primary sequences allowed the localization of probable active sites and residues involved in thermostability.

Original languageEnglish
Pages (from-to)503-506
Number of pages4
JournalApplied Microbiology and Biotechnology
Volume36
Issue number4
DOIs
Publication statusPublished - Jan 1992
Externally publishedYes

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