In vitro response to mitogens by duck splenic mononuclear cells

K. Vickery; A. Pajkos; Y. Cossart

doi:10.1016/0034-5288(95)90011-X

In vitro response to mitogens by duck splenic mononuclear cells

K. Vickery^*, A. Pajkos, Y. Cossart

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

This study has defined the effects of cell concentration, culture conditions and mitogen concentration on the uptake of ³H-labelled thymidine by mononuclear cells (SMC) purified from the spleens of ducks. The mitogen stimulation of cultures containing from 5 × 10² to 2 × 10⁶ cells per well indicated that the optimal concentration of duck SMC was 5 × 10⁵ cells per well. Cultures with concentrations of 1 × 10⁶ cells per well or more took up ³H-thymidine without the need for mitogenic stimulation. The response to each mitogen tested - phytohaemagglutinin (PHA), concanavalin A (ConA) and lipopolysaccharide (LPS) - was distinctive, with between duck variation in the time for peak stimulation, the level of stimulation and the mitogen concentration required for maximum stimulation. The response of the SMC of all the ducks was strongest to stimulation with PHA, the response to ConA was variable and the response to LPs was poor. Supplementation of the media with 20 per cent fetal calf serum instead of autologous duck serum was satisfactory.

Original language	English
Pages (from-to)	242-246
Number of pages	5
Journal	Research in Veterinary Science
Volume	59
Issue number	3
DOIs	https://doi.org/10.1016/0034-5288(95)90011-X
Publication status	Published - 1995
Externally published	Yes

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10.1016/0034-5288(95)90011-X

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title = "In vitro response to mitogens by duck splenic mononuclear cells",

abstract = "This study has defined the effects of cell concentration, culture conditions and mitogen concentration on the uptake of 3H-labelled thymidine by mononuclear cells (SMC) purified from the spleens of ducks. The mitogen stimulation of cultures containing from 5 × 102 to 2 × 106 cells per well indicated that the optimal concentration of duck SMC was 5 × 105 cells per well. Cultures with concentrations of 1 × 106 cells per well or more took up 3H-thymidine without the need for mitogenic stimulation. The response to each mitogen tested - phytohaemagglutinin (PHA), concanavalin A (ConA) and lipopolysaccharide (LPS) - was distinctive, with between duck variation in the time for peak stimulation, the level of stimulation and the mitogen concentration required for maximum stimulation. The response of the SMC of all the ducks was strongest to stimulation with PHA, the response to ConA was variable and the response to LPs was poor. Supplementation of the media with 20 per cent fetal calf serum instead of autologous duck serum was satisfactory.",

author = "K. Vickery and A. Pajkos and Y. Cossart",

year = "1995",

doi = "10.1016/0034-5288(95)90011-X",

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T1 - In vitro response to mitogens by duck splenic mononuclear cells

AU - Vickery, K.

AU - Pajkos, A.

AU - Cossart, Y.

PY - 1995

Y1 - 1995

N2 - This study has defined the effects of cell concentration, culture conditions and mitogen concentration on the uptake of 3H-labelled thymidine by mononuclear cells (SMC) purified from the spleens of ducks. The mitogen stimulation of cultures containing from 5 × 102 to 2 × 106 cells per well indicated that the optimal concentration of duck SMC was 5 × 105 cells per well. Cultures with concentrations of 1 × 106 cells per well or more took up 3H-thymidine without the need for mitogenic stimulation. The response to each mitogen tested - phytohaemagglutinin (PHA), concanavalin A (ConA) and lipopolysaccharide (LPS) - was distinctive, with between duck variation in the time for peak stimulation, the level of stimulation and the mitogen concentration required for maximum stimulation. The response of the SMC of all the ducks was strongest to stimulation with PHA, the response to ConA was variable and the response to LPs was poor. Supplementation of the media with 20 per cent fetal calf serum instead of autologous duck serum was satisfactory.

AB - This study has defined the effects of cell concentration, culture conditions and mitogen concentration on the uptake of 3H-labelled thymidine by mononuclear cells (SMC) purified from the spleens of ducks. The mitogen stimulation of cultures containing from 5 × 102 to 2 × 106 cells per well indicated that the optimal concentration of duck SMC was 5 × 105 cells per well. Cultures with concentrations of 1 × 106 cells per well or more took up 3H-thymidine without the need for mitogenic stimulation. The response to each mitogen tested - phytohaemagglutinin (PHA), concanavalin A (ConA) and lipopolysaccharide (LPS) - was distinctive, with between duck variation in the time for peak stimulation, the level of stimulation and the mitogen concentration required for maximum stimulation. The response of the SMC of all the ducks was strongest to stimulation with PHA, the response to ConA was variable and the response to LPs was poor. Supplementation of the media with 20 per cent fetal calf serum instead of autologous duck serum was satisfactory.

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DO - 10.1016/0034-5288(95)90011-X

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VL - 59

SP - 242

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JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 3

ER -

In vitro response to mitogens by duck splenic mononuclear cells

Abstract

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