In vivo validation of bimolecular fluorescence complementation (BiFC) to investigate aggregate formation in amyotrophic lateral sclerosis (ALS)

Emily K. Don, Alina Maschirow, Rowan A. W. Radford, Natalie M. Scherer, Andrés Vidal-Itriago, Alison Hogan, Cindy Maurel, Isabel Formella, Jack J. Stoddart, Thomas E. Hall, Albert Lee, Bingyang Shi, Nicholas J. Cole, Angela S. Laird, Andrew P. Badrock, Roger S. Chung, Marco Morsch

Research output: Contribution to journalArticlepeer-review

Abstract

Amyotrophic lateral sclerosis (ALS) is a form of motor neuron disease (MND) that is characterized by the progressive loss of motor neurons within the spinal cord, brainstem, and motor cortex. Although ALS clinically manifests as a heterogeneous disease, with varying disease onset and survival, a unifying feature is the presence of ubiquitinated cytoplasmic protein inclusion aggregates containing TDP-43. However, the precise mechanisms linking protein inclusions and aggregation to neuronal loss are currently poorly understood. Bimolecular fluorescence complementation (BiFC) takes advantage of the association of fluorophore fragments (non-fluorescent on their own) that are attached to an aggregation-prone protein of interest. Interaction of the proteins of interest allows for the fluorescent reporter protein to fold into its native state and emit a fluorescent signal. Here, we combined the power of BiFC with the advantages of the zebrafish system to validate, optimize, and visualize the formation of ALS-linked aggregates in real time in a vertebrate model. We further provide in vivo validation of the selectivity of this technique and demonstrate reduced spontaneous self-assembly of the non-fluorescent fragments in vivo by introducing a fluorophore mutation. Additionally, we report preliminary findings on the dynamic aggregation of the ALS-linked hallmark proteins Fus and TDP-43 in their corresponding nuclear and cytoplasmic compartments using BiFC. Overall, our data demonstrates the suitability of this BiFC approach to study and characterize ALS-linked aggregate formation in vivo. Importantly, the same principle can be applied in the context of other neurodegenerative diseases and has therefore critical implications to advance our understanding of pathologies that underlie aberrant protein aggregation.

Original languageEnglish
Pages (from-to)2061-2074
Number of pages14
JournalMolecular Neurobiology
Volume58
Issue number5
Early online date7 Jan 2021
DOIs
Publication statusPublished - May 2021

Bibliographical note

Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Aggregate formation
  • ALS
  • Bimolecular fluorescence complementation
  • FUS
  • TDP-43
  • Zebrafish

Fingerprint Dive into the research topics of 'In vivo validation of bimolecular fluorescence complementation (BiFC) to investigate aggregate formation in amyotrophic lateral sclerosis (ALS)'. Together they form a unique fingerprint.

Cite this