TY - JOUR
T1 - Increased expression of the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor by rat facial motoneurons after neonatal axotomy and CNTF treatment
AU - Duberley, R. M.
AU - Johnson, I. P.
PY - 1996/11/8
Y1 - 1996/11/8
N2 - Expression by rat motoneurons of mRNAs for the alpha subunit of the ciliary neurotrophic factor receptor (CNTFR alpha), receptor tyrosine kinase-C (trkC), calcitonin gene-related peptide (CGRP) and galanin was studied by in situ hybridisation, 6d after rat facial nerve transection on postnatal day 1. Similar numbers of motoneurons in both injured and non-injured nuclei hybridised with all probes, despite a reduction in total numbers of motoneurons ipsilaterally. When ciliary neurotrophic factor (CNTF) was applied to the proximal nerve, twice as many motoneurons in the injured nucleus hybridised with the CNTFR alpha probe ipsilaterally compared to the non-injured nucleus, while motoneuronal loss was not evident. These results indicate that survival of motoneurons after neonatal axotomy and CNTF treatment is associated with increased CNTF receptor alpha expression.
AB - Expression by rat motoneurons of mRNAs for the alpha subunit of the ciliary neurotrophic factor receptor (CNTFR alpha), receptor tyrosine kinase-C (trkC), calcitonin gene-related peptide (CGRP) and galanin was studied by in situ hybridisation, 6d after rat facial nerve transection on postnatal day 1. Similar numbers of motoneurons in both injured and non-injured nuclei hybridised with all probes, despite a reduction in total numbers of motoneurons ipsilaterally. When ciliary neurotrophic factor (CNTF) was applied to the proximal nerve, twice as many motoneurons in the injured nucleus hybridised with the CNTFR alpha probe ipsilaterally compared to the non-injured nucleus, while motoneuronal loss was not evident. These results indicate that survival of motoneurons after neonatal axotomy and CNTF treatment is associated with increased CNTF receptor alpha expression.
KW - Axotomy
KW - Ciliary neurotrophic factor receptor
KW - In situ hybridisation
KW - Neonate
UR - http://www.scopus.com/inward/record.url?scp=0030575921&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(96)13124-4
DO - 10.1016/S0304-3940(96)13124-4
M3 - Article
C2 - 8945760
AN - SCOPUS:0030575921
SN - 0304-3940
VL - 218
SP - 188
EP - 192
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -