Increased expression of the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor by rat facial motoneurons after neonatal axotomy and CNTF treatment

R. M. Duberley; I. P. Johnson

doi:10.1016/S0304-3940(96)13124-4

Increased expression of the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor by rat facial motoneurons after neonatal axotomy and CNTF treatment

R. M. Duberley, I. P. Johnson^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Expression by rat motoneurons of mRNAs for the alpha subunit of the ciliary neurotrophic factor receptor (CNTFR alpha), receptor tyrosine kinase-C (trkC), calcitonin gene-related peptide (CGRP) and galanin was studied by in situ hybridisation, 6d after rat facial nerve transection on postnatal day 1. Similar numbers of motoneurons in both injured and non-injured nuclei hybridised with all probes, despite a reduction in total numbers of motoneurons ipsilaterally. When ciliary neurotrophic factor (CNTF) was applied to the proximal nerve, twice as many motoneurons in the injured nucleus hybridised with the CNTFR alpha probe ipsilaterally compared to the non-injured nucleus, while motoneuronal loss was not evident. These results indicate that survival of motoneurons after neonatal axotomy and CNTF treatment is associated with increased CNTF receptor alpha expression.

Original language	English
Pages (from-to)	188-192
Number of pages	5
Journal	Neuroscience Letters
Volume	218
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(96)13124-4
Publication status	Published - 8 Nov 1996
Externally published	Yes

Keywords

Axotomy
Ciliary neurotrophic factor receptor
In situ hybridisation
Neonate

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10.1016/S0304-3940(96)13124-4

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title = "Increased expression of the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor by rat facial motoneurons after neonatal axotomy and CNTF treatment",

abstract = "Expression by rat motoneurons of mRNAs for the alpha subunit of the ciliary neurotrophic factor receptor (CNTFR alpha), receptor tyrosine kinase-C (trkC), calcitonin gene-related peptide (CGRP) and galanin was studied by in situ hybridisation, 6d after rat facial nerve transection on postnatal day 1. Similar numbers of motoneurons in both injured and non-injured nuclei hybridised with all probes, despite a reduction in total numbers of motoneurons ipsilaterally. When ciliary neurotrophic factor (CNTF) was applied to the proximal nerve, twice as many motoneurons in the injured nucleus hybridised with the CNTFR alpha probe ipsilaterally compared to the non-injured nucleus, while motoneuronal loss was not evident. These results indicate that survival of motoneurons after neonatal axotomy and CNTF treatment is associated with increased CNTF receptor alpha expression.",

keywords = "Axotomy, Ciliary neurotrophic factor receptor, In situ hybridisation, Neonate",

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T1 - Increased expression of the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor by rat facial motoneurons after neonatal axotomy and CNTF treatment

AU - Duberley, R. M.

AU - Johnson, I. P.

PY - 1996/11/8

Y1 - 1996/11/8

N2 - Expression by rat motoneurons of mRNAs for the alpha subunit of the ciliary neurotrophic factor receptor (CNTFR alpha), receptor tyrosine kinase-C (trkC), calcitonin gene-related peptide (CGRP) and galanin was studied by in situ hybridisation, 6d after rat facial nerve transection on postnatal day 1. Similar numbers of motoneurons in both injured and non-injured nuclei hybridised with all probes, despite a reduction in total numbers of motoneurons ipsilaterally. When ciliary neurotrophic factor (CNTF) was applied to the proximal nerve, twice as many motoneurons in the injured nucleus hybridised with the CNTFR alpha probe ipsilaterally compared to the non-injured nucleus, while motoneuronal loss was not evident. These results indicate that survival of motoneurons after neonatal axotomy and CNTF treatment is associated with increased CNTF receptor alpha expression.

AB - Expression by rat motoneurons of mRNAs for the alpha subunit of the ciliary neurotrophic factor receptor (CNTFR alpha), receptor tyrosine kinase-C (trkC), calcitonin gene-related peptide (CGRP) and galanin was studied by in situ hybridisation, 6d after rat facial nerve transection on postnatal day 1. Similar numbers of motoneurons in both injured and non-injured nuclei hybridised with all probes, despite a reduction in total numbers of motoneurons ipsilaterally. When ciliary neurotrophic factor (CNTF) was applied to the proximal nerve, twice as many motoneurons in the injured nucleus hybridised with the CNTFR alpha probe ipsilaterally compared to the non-injured nucleus, while motoneuronal loss was not evident. These results indicate that survival of motoneurons after neonatal axotomy and CNTF treatment is associated with increased CNTF receptor alpha expression.

KW - Axotomy

KW - Ciliary neurotrophic factor receptor

KW - In situ hybridisation

KW - Neonate

UR - https://www.scopus.com/pages/publications/0030575921

U2 - 10.1016/S0304-3940(96)13124-4

DO - 10.1016/S0304-3940(96)13124-4

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SN - 0304-3940

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JF - Neuroscience Letters

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ER -

Increased expression of the alpha subunit of the ciliary neurotrophic factor (CNTF) receptor by rat facial motoneurons after neonatal axotomy and CNTF treatment

Abstract

Keywords

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