Increased tachykinin receptor gene expression in asthmatic lung and its modulation by steroids

I. M. Adcock; M. Peters; C. Gelder; H. Shirasaki; C. R. Brown; P. J. Barnes

Increased tachykinin receptor gene expression in asthmatic lung and its modulation by steroids

I. M. Adcock^*, M. Peters, C. Gelder, H. Shirasaki, C. R. Brown, P. J. Barnes

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

133 Citations (Scopus)

Abstract

Substance P has several inflammatory effects on the airways mediated via neurokinin 1 receptors (NK₁Rs) and, if released from sensory nerves, may amplify the chronic inflammation seen in asthma. Northern blot analysis of NK₁R mRNA in lung showed a 52 ± 10% (S.E.M.; P<0.01) increase in mRNA in the asthmatic lung compared with non-asthmatic control tissue. NK₁R mRNA was reduced by 84.5 ± 1.9% after incubation with dexamethasone (1 μM) for 3 h (P<0.01). In contrast, NK₂R mRNA was unaltered in asthmatic lungs and dexamethasone treatment had no effect on the level of NK₂R mRNA. These results suggest that chronic inflammation in asthma may result in increased NK₁R gene expression and that this effect is reversed by glucocorticosteroids.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of Molecular Endocrinology
Volume	11
Issue number	1
Publication status	Published - 1993
Externally published	Yes

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abstract = "Substance P has several inflammatory effects on the airways mediated via neurokinin 1 receptors (NK1Rs) and, if released from sensory nerves, may amplify the chronic inflammation seen in asthma. Northern blot analysis of NK1R mRNA in lung showed a 52 ± 10% (S.E.M.; P<0.01) increase in mRNA in the asthmatic lung compared with non-asthmatic control tissue. NK1R mRNA was reduced by 84.5 ± 1.9% after incubation with dexamethasone (1 μM) for 3 h (P<0.01). In contrast, NK2R mRNA was unaltered in asthmatic lungs and dexamethasone treatment had no effect on the level of NK2R mRNA. These results suggest that chronic inflammation in asthma may result in increased NK1R gene expression and that this effect is reversed by glucocorticosteroids.",

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T1 - Increased tachykinin receptor gene expression in asthmatic lung and its modulation by steroids

AU - Adcock, I. M.

AU - Peters, M.

AU - Gelder, C.

AU - Shirasaki, H.

AU - Brown, C. R.

AU - Barnes, P. J.

PY - 1993

Y1 - 1993

N2 - Substance P has several inflammatory effects on the airways mediated via neurokinin 1 receptors (NK1Rs) and, if released from sensory nerves, may amplify the chronic inflammation seen in asthma. Northern blot analysis of NK1R mRNA in lung showed a 52 ± 10% (S.E.M.; P<0.01) increase in mRNA in the asthmatic lung compared with non-asthmatic control tissue. NK1R mRNA was reduced by 84.5 ± 1.9% after incubation with dexamethasone (1 μM) for 3 h (P<0.01). In contrast, NK2R mRNA was unaltered in asthmatic lungs and dexamethasone treatment had no effect on the level of NK2R mRNA. These results suggest that chronic inflammation in asthma may result in increased NK1R gene expression and that this effect is reversed by glucocorticosteroids.

AB - Substance P has several inflammatory effects on the airways mediated via neurokinin 1 receptors (NK1Rs) and, if released from sensory nerves, may amplify the chronic inflammation seen in asthma. Northern blot analysis of NK1R mRNA in lung showed a 52 ± 10% (S.E.M.; P<0.01) increase in mRNA in the asthmatic lung compared with non-asthmatic control tissue. NK1R mRNA was reduced by 84.5 ± 1.9% after incubation with dexamethasone (1 μM) for 3 h (P<0.01). In contrast, NK2R mRNA was unaltered in asthmatic lungs and dexamethasone treatment had no effect on the level of NK2R mRNA. These results suggest that chronic inflammation in asthma may result in increased NK1R gene expression and that this effect is reversed by glucocorticosteroids.

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Increased tachykinin receptor gene expression in asthmatic lung and its modulation by steroids

Abstract

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