Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 ng/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0 ng/g fresh wt.). The analysis was carried out using a stable isotope dilution assay in combination with gas chromatography-mass spectrometry. This is the first unambiguous determination of endogenous IAA in genetic tumour tissues.
- gas chromatography-mass spectrometry
- genetic tumours
- indole-3-acetic acid
- stable isotope dilution