Indole-3-acetic acid determination in cultured crown-gall and genetic tumour tissues by gas chromatography-mass spectrometry

L. M S Palni; R. E. Summons

doi:10.1007/BF00035019

Indole-3-acetic acid determination in cultured crown-gall and genetic tumour tissues by gas chromatography-mass spectrometry

L. M S Palni^*, R. E. Summons

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 ng/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0 ng/g fresh wt.). The analysis was carried out using a stable isotope dilution assay in combination with gas chromatography-mass spectrometry. This is the first unambiguous determination of endogenous IAA in genetic tumour tissues.

Original language	English
Pages (from-to)	51-57
Number of pages	7
Journal	Plant Growth Regulation
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/BF00035019
Publication status	Published - Jan 1987

Keywords

Crown-gall
gas chromatography-mass spectrometry
genetic tumours
indole-3-acetic acid
stable isotope dilution

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10.1007/BF00035019

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abstract = "Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 ng/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0 ng/g fresh wt.). The analysis was carried out using a stable isotope dilution assay in combination with gas chromatography-mass spectrometry. This is the first unambiguous determination of endogenous IAA in genetic tumour tissues.",

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author = "Palni, {L. M S} and Summons, {R. E.}",

year = "1987",

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AU - Palni, L. M S

AU - Summons, R. E.

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N2 - Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 ng/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0 ng/g fresh wt.). The analysis was carried out using a stable isotope dilution assay in combination with gas chromatography-mass spectrometry. This is the first unambiguous determination of endogenous IAA in genetic tumour tissues.

AB - Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 ng/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0 ng/g fresh wt.). The analysis was carried out using a stable isotope dilution assay in combination with gas chromatography-mass spectrometry. This is the first unambiguous determination of endogenous IAA in genetic tumour tissues.

KW - Crown-gall

KW - gas chromatography-mass spectrometry

KW - genetic tumours

KW - indole-3-acetic acid

KW - stable isotope dilution

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U2 - 10.1007/BF00035019

DO - 10.1007/BF00035019

M3 - Article

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VL - 5

SP - 51

EP - 57

JO - Plant Growth Regulation

JF - Plant Growth Regulation

IS - 1

ER -

Indole-3-acetic acid determination in cultured crown-gall and genetic tumour tissues by gas chromatography-mass spectrometry

Abstract

Keywords

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