Indole-3-acetic acid determination in cultured crown-gall and genetic tumour tissues by gas chromatography-mass spectrometry

L. M S Palni*, R. E. Summons

*Corresponding author for this work

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 ng/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0 ng/g fresh wt.). The analysis was carried out using a stable isotope dilution assay in combination with gas chromatography-mass spectrometry. This is the first unambiguous determination of endogenous IAA in genetic tumour tissues.

    Original languageEnglish
    Pages (from-to)51-57
    Number of pages7
    JournalPlant Growth Regulation
    Volume5
    Issue number1
    DOIs
    Publication statusPublished - Jan 1987

    Keywords

    • Crown-gall
    • gas chromatography-mass spectrometry
    • genetic tumours
    • indole-3-acetic acid
    • stable isotope dilution

    Fingerprint Dive into the research topics of 'Indole-3-acetic acid determination in cultured crown-gall and genetic tumour tissues by gas chromatography-mass spectrometry'. Together they form a unique fingerprint.

  • Cite this