Indole-3-acetic acid determination in cultured crown-gall and genetic tumour tissues by gas chromatography-mass spectrometry

L. M S Palni*, R. E. Summons

*Corresponding author for this work

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Indole-3-acetic acid (IAA) was identified and quantified in three cultured tumour lines, which included crown-gall tissue of Datura (25.4 ng/g fresh wt.) and two genetic tumour lines of tobacco (4.6 and 8.0 ng/g fresh wt.). The analysis was carried out using a stable isotope dilution assay in combination with gas chromatography-mass spectrometry. This is the first unambiguous determination of endogenous IAA in genetic tumour tissues.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalPlant Growth Regulation
Issue number1
Publication statusPublished - Jan 1987



  • Crown-gall
  • gas chromatography-mass spectrometry
  • genetic tumours
  • indole-3-acetic acid
  • stable isotope dilution

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