TY - JOUR
T1 - Inducibility and stability of auxotrophic mutations in Mycobacterium fortuitum, M. smegmatis and M. vaccae
AU - Subramanyam, V. R.
AU - Pal, B. B.
AU - Mohanty, Keshari K.
PY - 1989
Y1 - 1989
N2 - Stable auxotrophic mutants were isolated by N‐methyl‐N'‐nitro‐N‐nitrosoguanidine treatment of Mycobacterium fortuitum NIHJ 1615, M. smegmatis NIHJ 1628 and M. vaccae NIHJ 1637. The number of stable mutants obtained were 0.17, 0.46 and 0.02% of the surviving mutagenized cells screened for the three species, respectively. Mutants differed from their parents in a single nutritional requirement except in the case of SM29, a mutant of M. fortuitum, and SM33, a mutant of M. smegmatis, which differed from their parents in two auxotrophic traits.
AB - Stable auxotrophic mutants were isolated by N‐methyl‐N'‐nitro‐N‐nitrosoguanidine treatment of Mycobacterium fortuitum NIHJ 1615, M. smegmatis NIHJ 1628 and M. vaccae NIHJ 1637. The number of stable mutants obtained were 0.17, 0.46 and 0.02% of the surviving mutagenized cells screened for the three species, respectively. Mutants differed from their parents in a single nutritional requirement except in the case of SM29, a mutant of M. fortuitum, and SM33, a mutant of M. smegmatis, which differed from their parents in two auxotrophic traits.
UR - http://www.scopus.com/inward/record.url?scp=0024348666&partnerID=8YFLogxK
U2 - 10.1111/j.1472-765X.1989.tb00238.x
DO - 10.1111/j.1472-765X.1989.tb00238.x
M3 - Article
AN - SCOPUS:0024348666
SN - 0266-8254
VL - 8
SP - 161
EP - 164
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
IS - 5
ER -