Stable auxotrophic mutants were isolated by N‐methyl‐N'‐nitro‐N‐nitrosoguanidine treatment of Mycobacterium fortuitum NIHJ 1615, M. smegmatis NIHJ 1628 and M. vaccae NIHJ 1637. The number of stable mutants obtained were 0.17, 0.46 and 0.02% of the surviving mutagenized cells screened for the three species, respectively. Mutants differed from their parents in a single nutritional requirement except in the case of SM29, a mutant of M. fortuitum, and SM33, a mutant of M. smegmatis, which differed from their parents in two auxotrophic traits.
|Number of pages||4|
|Journal||Letters in Applied Microbiology|
|Publication status||Published - 1989|