Induction of neural progenitor-like cells from human fibroblasts via a genetic material-free approach

Fahimeh Mirakhori, Bahman Zeynali, Hassan Rassouli, Ebrahim Shahbazi, Shiva Hashemizadeh, Sahar Kiani, Ghasem Hosseini Salekdeh, Hossein Baharvand

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)
2 Downloads (Pure)

Abstract

Background: A number of studies generated induced neural progenitor cells (iNPCs) from human fibroblasts by viral delivering defined transcription factors. However, the potential risks associated with gene delivery systems have limited their clinical use. We propose it would be safer to induce neural progenitor-like cells from human adult fibroblasts via a direct non-genetic alternative approach. 

Methodology/Principal Findings: Here, we have reported that seven rounds of TAT-SOX2 protein transduction in a defined chemical cocktail under a 3D sphere culture gradually morphed fibroblasts into neuroepithe-lial-like colonies. We were able to expand these cells for up to 20 passages. These cells could give rise to cells that expressed neurons and glia cell markers both in vitro and in vivo. 

Conclusions/Significance: These results show that our approach is beneficial for the genetic material-free generation of iNPCs from human fibroblasts where small chemical molecules can provide a valuable, viable strategy to boost and improve induction in a 3D sphere culture.

Original languageEnglish
Article numbere0135479
Pages (from-to)1-17
Number of pages17
JournalPLoS ONE
Volume10
Issue number8
DOIs
Publication statusPublished - 12 Aug 2015
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2015. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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