Influence of surface composition on the colloidal stability of ultra-small detonation nanodiamonds in biological media

Carlo Bradac, Ishan Das Rastogi, Nicole M. Cordina, Alfonso Garcia-Bennett, Louise J. Brown

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Fluorescent nanodiamonds (NDs) are strong contenders as bio-labels for life science imaging, diagnostics and therapeutics. Ultimately, for their use in biomedical applications, their size should ideally be <10 nm. Yet, even more critical for their specificity and efficient uptake in cellular systems, is their resilience to aggregation, which is dictated by their colloidal stability in complex, physiological environments. To this end, we characterize small detonation NDs (~5 nm) by examining their surface chemical profiles and stability in solutions of varying ionic strength and pH. Using dynamic light scattering measurements, we demonstrate that small monodisperse ND particles with chemically homogeneous and negatively charged surface profiles are more stable than positive particles under a broad range of simulated biological environments. We show that the colloidal stability of small clusters of both positive and negative detonation NDs is improved by functionalization with bovine serum albumin. Based on these analyses, we propose and describe strategies for enhancing the overall colloidal stability of detonation NDs and their resilience to aggregation. Our findings provide a practical framework towards the reduction in size of the bio-conjugates employed to probe complex biological systems, and the advancement of bio-imaging techniques with minimal perturbation of the molecular trafficking in cellular and organelle systems.

LanguageEnglish
Pages38-45
Number of pages8
JournalDiamond and Related Materials
Volume83
DOIs
Publication statusPublished - 1 Mar 2018

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Nanodiamonds
Detonation
detonation
Surface structure
resilience
Agglomeration
organelles
life sciences
Imaging techniques
profiles
albumins
imaging techniques
serums
Dynamic light scattering
Biological systems
Bovine Serum Albumin
Ionic strength
light scattering
Labels
perturbation

Cite this

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title = "Influence of surface composition on the colloidal stability of ultra-small detonation nanodiamonds in biological media",
abstract = "Fluorescent nanodiamonds (NDs) are strong contenders as bio-labels for life science imaging, diagnostics and therapeutics. Ultimately, for their use in biomedical applications, their size should ideally be <10 nm. Yet, even more critical for their specificity and efficient uptake in cellular systems, is their resilience to aggregation, which is dictated by their colloidal stability in complex, physiological environments. To this end, we characterize small detonation NDs (~5 nm) by examining their surface chemical profiles and stability in solutions of varying ionic strength and pH. Using dynamic light scattering measurements, we demonstrate that small monodisperse ND particles with chemically homogeneous and negatively charged surface profiles are more stable than positive particles under a broad range of simulated biological environments. We show that the colloidal stability of small clusters of both positive and negative detonation NDs is improved by functionalization with bovine serum albumin. Based on these analyses, we propose and describe strategies for enhancing the overall colloidal stability of detonation NDs and their resilience to aggregation. Our findings provide a practical framework towards the reduction in size of the bio-conjugates employed to probe complex biological systems, and the advancement of bio-imaging techniques with minimal perturbation of the molecular trafficking in cellular and organelle systems.",
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Influence of surface composition on the colloidal stability of ultra-small detonation nanodiamonds in biological media. / Bradac, Carlo; Rastogi, Ishan Das; Cordina, Nicole M.; Garcia-Bennett, Alfonso; Brown, Louise J.

In: Diamond and Related Materials, Vol. 83, 01.03.2018, p. 38-45.

Research output: Contribution to journalArticleResearchpeer-review

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