Inhibitory effect of a French maritime pine bark extract-based nutritional supplement on TNF-α-induced inflammation and oxidative stress in human coronary artery endothelial cells

Kristine C. Y. McGrath, Sumudu V. S. Gangoda, Xiao-Hong Li, Lucinda S. McRobb, Alison K. Heather

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)
    203 Downloads (Pure)

    Abstract

    Oxidative stress and inflammation, leading to endothelial dysfunction, contribute to the pathogenesis of atherosclerosis. The popularity of natural product supplements has increased in recent years, especially those with purported anti-inflammatory and/or antioxidant effects. The efficacy and mechanism of many of these products are not yet well understood. In this study, we tested the antioxidant and anti-inflammatory effects of a supplement, HIPER Health Supplement (HIPER), on cytokine-induced inflammation and oxidative stress in human coronary artery endothelial cells (HCAECs). HIPER is a mixture of French maritime pine bark extract (PBE), honey, aloe vera, and papaya extract. Treatment for 24 hours with HIPER reduced TNF-α-induced reactive oxygen species (ROS) generation that was associated with decreased NADPH oxidase 4 and increased superoxide dismutase-1 expression. HIPER inhibited TNF-α induced monocyte adhesion to HCAECs that was in keeping with decreased expression of vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1 and decreased nuclear factor-kappa B (NF-B) activation. Further investigation of mechanism showed HIPER reduced TNF-α induced IBα and p38 and MEK1/2 MAP kinases phosphorylation. Our findings show that HIPER has potent inhibitory effects on HCAECs inflammatory and oxidative stress responses that may protect against endothelial dysfunction that underlies early atherosclerotic lesion formation.

    Original languageEnglish
    Article number260530
    Pages (from-to)1-7
    Number of pages7
    JournalEvidence-based Complementary and Alternative Medicine
    Volume2015
    DOIs
    Publication statusPublished - 2015

    Bibliographical note

    Copyright the Author(s) 2015. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

    A corrigendum exists for this article, and can be found in Evidence-Based Complementary and Alternative Medicine, Volume 2018, Article ID 5642129 at doi: 10.1155/2018/5642129

    Fingerprint

    Dive into the research topics of 'Inhibitory effect of a French maritime pine bark extract-based nutritional supplement on TNF-α-induced inflammation and oxidative stress in human coronary artery endothelial cells'. Together they form a unique fingerprint.

    Cite this