Insights on virulence from the complete genome of Staphylococcus capitis

David R. Cameron, Jhih Hang Jiang, Karl A. Hassan, Liam D.H. Elbourne, Kellie L. Tuck, Ian T. Paulsen, Anton Y. Peleg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)
20 Downloads (Pure)

Abstract

Staphylococcus capitis is an opportunistic pathogen of the coagulase negative staphylococci (CoNS). Functional genomic studies of S. capitis have thus far been limited by a lack of available complete genome sequences. Here, we determined the closed S. capitis genome and methylome using Single Molecule Real Time (SMRT) sequencing. The strain, AYP1020, harbors a single circular chromosome of 2.44 Mb encoding 2304 predicted proteins, which is the smallest of all complete staphylococcal genomes sequenced to date. AYP1020 harbors two large mobile genetic elements; a plasmid designated pAYP1020 (59.6 Kb) and a prophage, ΦAYP1020 (48.5 Kb). Methylome analysis identified significant adenine methylation across the genome involving two distinct methylation motifs (1972 putative 6-methyladenine (m6A) residues identified). Putative adenine methyltransferases were also identified. Comparative analysis of AYP1020 and the closely related CoNS, S. epidermidis RP62a, revealed a host of virulence factors that likely contribute to S. capitis pathogenicity, most notably genes important for biofilm formation and a suite of phenol soluble modulins (PSMs); the expression/production of these factors were corroborated by functional assays. The complete S. capitis genome will aid future studies on the evolution and pathogenesis of the coagulase negative staphylococci.

Original languageEnglish
Article number980
Pages (from-to)1-12
Number of pages12
JournalFrontiers in Microbiology
Volume6
DOIs
Publication statusPublished - 23 Sep 2015

Bibliographical note

Copyright the Author(s) 2015. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Coagulase-negative staphylococci
  • Cons
  • Genomics
  • Methylation
  • SMRT sequencing

Fingerprint Dive into the research topics of 'Insights on virulence from the complete genome of Staphylococcus capitis'. Together they form a unique fingerprint.

Cite this