TY - JOUR
T1 - Integrated proteomics identified up-regulated focal adhesion-mediated proteins in human squamous cell carcinoma in an orthotopic murine model
AU - Granato, Daniela C.
AU - Zanetti, Mariana R.
AU - Kawahara, Rebeca
AU - Yokoo, Sami
AU - Domingues, Romenia R.
AU - Aragao, Annelize Z.
AU - Agostini, Michelle
AU - Carazzolle, Marcelo F.
AU - Vidal, Ramon O.
AU - Flores, Isadora L.
AU - Korvala, Johanna
AU - Cervigne, Nilva K.
AU - Silva, Alan R. S.
AU - Coletta, Ricardo D.
AU - Graner, Edgard
AU - Sherman, Nicholas E.
AU - Paes Leme, Adriana F.
N1 - Copyright the Author(s) 2014. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2014/5/23
Y1 - 2014/5/23
N2 - Understanding the molecular mechanisms of oral carcinogenesis will yield important advances in diagnostics, prognostics, effective treatment, and outcome of oral cancer. Hence, in this study we have investigated the proteomic and peptidomic profiles by combining an orthotopic murine model of oral squamous cell carcinoma (OSCC), mass spectrometry-based proteomics and biological network analysis. Our results indicated the up-regulation of proteins involved in actin cytoskeleton organization and cell-cell junction assembly events and their expression was validated in human OSCC tissues. In addition, the functional relevance of talin-1 in OSCC adhesion, migration and invasion was demonstrated. Taken together, this study identified specific processes deregulated in oral cancer and provided novel refined OSCC-targeting molecules.
AB - Understanding the molecular mechanisms of oral carcinogenesis will yield important advances in diagnostics, prognostics, effective treatment, and outcome of oral cancer. Hence, in this study we have investigated the proteomic and peptidomic profiles by combining an orthotopic murine model of oral squamous cell carcinoma (OSCC), mass spectrometry-based proteomics and biological network analysis. Our results indicated the up-regulation of proteins involved in actin cytoskeleton organization and cell-cell junction assembly events and their expression was validated in human OSCC tissues. In addition, the functional relevance of talin-1 in OSCC adhesion, migration and invasion was demonstrated. Taken together, this study identified specific processes deregulated in oral cancer and provided novel refined OSCC-targeting molecules.
UR - http://www.scopus.com/inward/record.url?scp=84901473166&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0098208
DO - 10.1371/journal.pone.0098208
M3 - Article
C2 - 24858105
SN - 1932-6203
VL - 9
JO - PLoS ONE
JF - PLoS ONE
IS - 5
M1 - e98208
ER -