Intelligent image-activated cell sorting

Nao Nitta; Takeaki Sugimura; Akihiro Isozaki; Hideharu Mikami; Kei Hiraki; Shinya Sakuma; Takanori Iino; Fumihito Arai; Taichiro Endo; Yasuhiro Fujiwaki; Hideya Fukuzawa; Misa Hase; Takeshi Hayakawa; Kotaro Hiramatsu; Yu Hoshino; Mary Inaba; Takuro Ito; Hiroshi Karakawa; Yusuke Kasai; Kenichi Koizumi; SangWook Lee; Cheng Lei; Ming Li; Takanori Maeno; Satoshi Matsusaka; Daichi Murakami; Atsuhiro Nakagawa; Yusuke Oguchi; Minoru Oikawa; Tadataka Ota; Kiyotaka Shiba; Hirofumi Shintaku; Yoshitaka Shirasaki; Kanako Suga; Yutaka Suzuki; Nobutake Suzuki; Yo Tanaka; Hiroshi Tezuka; Chihana Toyokawa; Yaxiaer Yalikun; Makoto Yamada; Mai Yamagishi; Takashi Yamanouchi; Atsushi Yasumoto; Yutaka Yatomi; Masayuki Yazawa; Dino Di Carlo; Yoichiroh Hosokawa; Sotaro Uemura; Yasuyuki Ozeki; Keisuke Goda

doi:10.1016/j.cell.2018.08.028

Intelligent image-activated cell sorting

Nao Nitta, Takeaki Sugimura, Akihiro Isozaki, Hideharu Mikami, Kei Hiraki, Shinya Sakuma, Takanori Iino, Fumihito Arai, Taichiro Endo, Yasuhiro Fujiwaki, Hideya Fukuzawa, Misa Hase, Takeshi Hayakawa, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Kenichi KoizumiSangWook Lee, Cheng Lei, Ming Li, Takanori Maeno, Satoshi Matsusaka, Daichi Murakami, Atsuhiro Nakagawa, Yusuke Oguchi, Minoru Oikawa, Tadataka Ota, Kiyotaka Shiba, Hirofumi Shintaku, Yoshitaka Shirasaki, Kanako Suga, Yutaka Suzuki, Nobutake Suzuki, Yo Tanaka, Hiroshi Tezuka, Chihana Toyokawa, Yaxiaer Yalikun, Makoto Yamada, Mai Yamagishi, Takashi Yamanouchi, Atsushi Yasumoto, Yutaka Yatomi, Masayuki Yazawa, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Yasuyuki Ozeki, Keisuke Goda

Research output: Contribution to journal › Article › peer-review

289 Citations (Scopus)

Abstract

A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.

Original language	English
Pages (from-to)	266-276
Number of pages	11
Journal	Cell
Volume	175
Issue number	1
DOIs	https://doi.org/10.1016/j.cell.2018.08.028
Publication status	Published - 20 Sep 2018
Externally published	Yes

Bibliographical note

Plus 8 pages STAR★Methods (e1-e8) and 5 non-pagiated supplemental figures.

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10.1016/j.cell.2018.08.028

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Nitta, Nao ; Sugimura, Takeaki ; Isozaki, Akihiro ; Mikami, Hideharu ; Hiraki, Kei ; Sakuma, Shinya ; Iino, Takanori ; Arai, Fumihito ; Endo, Taichiro ; Fujiwaki, Yasuhiro ; Fukuzawa, Hideya ; Hase, Misa ; Hayakawa, Takeshi ; Hiramatsu, Kotaro ; Hoshino, Yu ; Inaba, Mary ; Ito, Takuro ; Karakawa, Hiroshi ; Kasai, Yusuke ; Koizumi, Kenichi ; Lee, SangWook ; Lei, Cheng ; Li, Ming ; Maeno, Takanori ; Matsusaka, Satoshi ; Murakami, Daichi ; Nakagawa, Atsuhiro ; Oguchi, Yusuke ; Oikawa, Minoru ; Ota, Tadataka ; Shiba, Kiyotaka ; Shintaku, Hirofumi ; Shirasaki, Yoshitaka ; Suga, Kanako ; Suzuki, Yutaka ; Suzuki, Nobutake ; Tanaka, Yo ; Tezuka, Hiroshi ; Toyokawa, Chihana ; Yalikun, Yaxiaer ; Yamada, Makoto ; Yamagishi, Mai ; Yamanouchi, Takashi ; Yasumoto, Atsushi ; Yatomi, Yutaka ; Yazawa, Masayuki ; Di Carlo, Dino ; Hosokawa, Yoichiroh ; Uemura, Sotaro ; Ozeki, Yasuyuki ; Goda, Keisuke. / Intelligent image-activated cell sorting. In: Cell. 2018 ; Vol. 175, No. 1. pp. 266-276.

@article{2bc9a2eb8dc34cc5ac2049cda9956da5,

title = "Intelligent image-activated cell sorting",

abstract = "A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.",

author = "Nao Nitta and Takeaki Sugimura and Akihiro Isozaki and Hideharu Mikami and Kei Hiraki and Shinya Sakuma and Takanori Iino and Fumihito Arai and Taichiro Endo and Yasuhiro Fujiwaki and Hideya Fukuzawa and Misa Hase and Takeshi Hayakawa and Kotaro Hiramatsu and Yu Hoshino and Mary Inaba and Takuro Ito and Hiroshi Karakawa and Yusuke Kasai and Kenichi Koizumi and SangWook Lee and Cheng Lei and Ming Li and Takanori Maeno and Satoshi Matsusaka and Daichi Murakami and Atsuhiro Nakagawa and Yusuke Oguchi and Minoru Oikawa and Tadataka Ota and Kiyotaka Shiba and Hirofumi Shintaku and Yoshitaka Shirasaki and Kanako Suga and Yutaka Suzuki and Nobutake Suzuki and Yo Tanaka and Hiroshi Tezuka and Chihana Toyokawa and Yaxiaer Yalikun and Makoto Yamada and Mai Yamagishi and Takashi Yamanouchi and Atsushi Yasumoto and Yutaka Yatomi and Masayuki Yazawa and {Di Carlo}, Dino and Yoichiroh Hosokawa and Sotaro Uemura and Yasuyuki Ozeki and Keisuke Goda",

note = "Plus 8 pages STAR★Methods (e1-e8) and 5 non-pagiated supplemental figures.",

year = "2018",

month = sep,

day = "20",

doi = "10.1016/j.cell.2018.08.028",

language = "English",

volume = "175",

pages = "266--276",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

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Nitta, N, Sugimura, T, Isozaki, A, Mikami, H, Hiraki, K, Sakuma, S, Iino, T, Arai, F, Endo, T, Fujiwaki, Y, Fukuzawa, H, Hase, M, Hayakawa, T, Hiramatsu, K, Hoshino, Y, Inaba, M, Ito, T, Karakawa, H, Kasai, Y, Koizumi, K, Lee, S, Lei, C, Li, M, Maeno, T, Matsusaka, S, Murakami, D, Nakagawa, A, Oguchi, Y, Oikawa, M, Ota, T, Shiba, K, Shintaku, H, Shirasaki, Y, Suga, K, Suzuki, Y, Suzuki, N, Tanaka, Y, Tezuka, H, Toyokawa, C, Yalikun, Y, Yamada, M, Yamagishi, M, Yamanouchi, T, Yasumoto, A, Yatomi, Y, Yazawa, M, Di Carlo, D, Hosokawa, Y, Uemura, S, Ozeki, Y & Goda, K 2018, 'Intelligent image-activated cell sorting', Cell, vol. 175, no. 1, pp. 266-276. https://doi.org/10.1016/j.cell.2018.08.028

Intelligent image-activated cell sorting. / Nitta, Nao; Sugimura, Takeaki; Isozaki, Akihiro; Mikami, Hideharu; Hiraki, Kei; Sakuma, Shinya; Iino, Takanori; Arai, Fumihito; Endo, Taichiro; Fujiwaki, Yasuhiro; Fukuzawa, Hideya; Hase, Misa; Hayakawa, Takeshi; Hiramatsu, Kotaro; Hoshino, Yu; Inaba, Mary; Ito, Takuro; Karakawa, Hiroshi; Kasai, Yusuke; Koizumi, Kenichi; Lee, SangWook; Lei, Cheng; Li, Ming; Maeno, Takanori; Matsusaka, Satoshi; Murakami, Daichi; Nakagawa, Atsuhiro; Oguchi, Yusuke; Oikawa, Minoru; Ota, Tadataka; Shiba, Kiyotaka; Shintaku, Hirofumi; Shirasaki, Yoshitaka; Suga, Kanako; Suzuki, Yutaka; Suzuki, Nobutake; Tanaka, Yo; Tezuka, Hiroshi; Toyokawa, Chihana; Yalikun, Yaxiaer; Yamada, Makoto; Yamagishi, Mai; Yamanouchi, Takashi; Yasumoto, Atsushi; Yatomi, Yutaka; Yazawa, Masayuki; Di Carlo, Dino; Hosokawa, Yoichiroh; Uemura, Sotaro; Ozeki, Yasuyuki; Goda, Keisuke.

In: Cell, Vol. 175, No. 1, 20.09.2018, p. 266-276.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Intelligent image-activated cell sorting

AU - Nitta, Nao

AU - Sugimura, Takeaki

AU - Isozaki, Akihiro

AU - Mikami, Hideharu

AU - Hiraki, Kei

AU - Sakuma, Shinya

AU - Iino, Takanori

AU - Arai, Fumihito

AU - Endo, Taichiro

AU - Fujiwaki, Yasuhiro

AU - Fukuzawa, Hideya

AU - Hase, Misa

AU - Hayakawa, Takeshi

AU - Hiramatsu, Kotaro

AU - Hoshino, Yu

AU - Inaba, Mary

AU - Ito, Takuro

AU - Karakawa, Hiroshi

AU - Kasai, Yusuke

AU - Koizumi, Kenichi

AU - Lee, SangWook

AU - Lei, Cheng

AU - Li, Ming

AU - Maeno, Takanori

AU - Matsusaka, Satoshi

AU - Murakami, Daichi

AU - Nakagawa, Atsuhiro

AU - Oguchi, Yusuke

AU - Oikawa, Minoru

AU - Ota, Tadataka

AU - Shiba, Kiyotaka

AU - Shintaku, Hirofumi

AU - Shirasaki, Yoshitaka

AU - Suga, Kanako

AU - Suzuki, Yutaka

AU - Suzuki, Nobutake

AU - Tanaka, Yo

AU - Tezuka, Hiroshi

AU - Toyokawa, Chihana

AU - Yalikun, Yaxiaer

AU - Yamada, Makoto

AU - Yamagishi, Mai

AU - Yamanouchi, Takashi

AU - Yasumoto, Atsushi

AU - Yatomi, Yutaka

AU - Yazawa, Masayuki

AU - Di Carlo, Dino

AU - Hosokawa, Yoichiroh

AU - Uemura, Sotaro

AU - Ozeki, Yasuyuki

AU - Goda, Keisuke

N1 - Plus 8 pages STAR★Methods (e1-e8) and 5 non-pagiated supplemental figures.

PY - 2018/9/20

Y1 - 2018/9/20

N2 - A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.

AB - A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.

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U2 - 10.1016/j.cell.2018.08.028

DO - 10.1016/j.cell.2018.08.028

M3 - Article

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VL - 175

SP - 266

EP - 276

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

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Intelligent image-activated cell sorting

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