TY - JOUR
T1 - Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
AU - Percy, Andrew J.
AU - Tamura-Wells, Jessica
AU - Albar, Juan Pablo
AU - Aloria, Kerman
AU - Amirkhani, Ardeshir
AU - Araujo, Gabriel D T
AU - Arizmendi, Jesus M.
AU - Blanco, Francisco J.
AU - Canals, Francesc
AU - Cho, Jin Young
AU - Colomé-Calls, Núria
AU - Corrales, Fernando J.
AU - Domont, Gilberto
AU - Espadas, Guadalupe
AU - Fernandez-Puente, Patricia
AU - Gil, Concha
AU - Haynes, Paul A.
AU - Hernáez, Maria Luisa
AU - Kim, Jin Young
AU - Kopylov, Arthur
AU - Marcilla, Miguel
AU - McKay, Mathew J.
AU - Mirzaei, Mehdi
AU - Molloy, Mark P.
AU - Ohlund, Leanne B.
AU - Paik, Young Ki
AU - Paradela, Alberto
AU - Raftery, Mark
AU - Sabidó, Eduard
AU - Sleno, Lekha
AU - Wilffert, Daniel
AU - Wolters, Justina C.
AU - Yoo, Jong Shin
AU - Zgoda, Victor
AU - Parker, Carol E.
AU - Borchers, Christoph H.
N1 - Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC-MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC-MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.
AB - The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC-MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC-MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.
UR - http://www.scopus.com/inward/record.url?scp=84940917784&partnerID=8YFLogxK
U2 - 10.1016/j.euprot.2015.06.001
DO - 10.1016/j.euprot.2015.06.001
M3 - Article
AN - SCOPUS:84940917784
SN - 2212-9685
VL - 8
SP - 6
EP - 15
JO - EuPA Open Proteomics
JF - EuPA Open Proteomics
ER -