Interactions between horizontally acquired genes create a fitness cost in Pseudomonas aeruginosa

Alvaro San Millan*, Macarena Toll-Riera, Qin Qi, R. Craig MacLean

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Horizontal gene transfer (HGT) plays a key role in bacterial evolution, especially with respect to antibiotic resistance. Fitness costs associated with mobile genetic elements (MGEs) are thought to constrain HGT, but our understanding of these costs remains fragmentary, making it difficult to predict the success of HGT events. Here we use the interaction between P. aeruginosa and a costly plasmid (pNUK73) to investigate the molecular basis of the cost of HGT. Using RNA-Seq, we show that the acquisition of pNUK73 results in a profound alteration of the transcriptional profile of chromosomal genes. Mutations that inactivate two genes encoded on chromosomally integrated MGEs recover these fitness costs and transcriptional changes by decreasing the expression of the pNUK73 replication gene. Our study demonstrates that interactions between MGEs can compromise bacterial fitness via altered gene expression, and we argue that conflicts between mobile elements impose a general constraint on evolution by HGT.

Original languageEnglish
Article number6845
Number of pages8
JournalNature Communications
Volume6
DOIs
Publication statusPublished - 21 Apr 2015
Externally publishedYes

Bibliographical note

Copyright the Publisher 2015. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Bacterial Proteins/genetics
  • Cloning, Molecular
  • Gene Expression Regulation, Bacterial/physiology
  • Gene Transfer, Horizontal
  • Genetic Fitness
  • Plasmids
  • Pseudomonas aeruginosa
  • RNA, Bacterial/genetics

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