Interactions of an emerging fungal pathogen Scedosporium aurantiacum with human lung epithelial cells

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Scedosporium fungi are found in various natural and host-associated environments, including the lungs of cystic fibrosis patients. However, their role in infection development remains underexplored. Here the attachment of conidia of a virulent S. aurantiacum strain WM 06.482 onto the human lung epithelial A549 cells in vitro was visualized using microscopy to examine the initial steps of infection. We showed that 75–80% of fungal conidia were bound to the A549 cells within four hours of co-incubation, and started to produce germ tubes. The germinating conidia seemed to invade the cells through the intercellular space, no intracellular uptake of fungal conidia by the airway epithelial cells after conidial attachment. Transcriptomic analysis of the A549 cells revealed that the up-regulated genes were mainly associated with cell repair and inflammatory processes indicating a protective response against S. aurantiacum infection. Network analysis of the differentially expressed genes showed activation of the innate immune system (NF-kB pathway) leading to the release of pro-inflammatory cytokines. We believe this is the first report showing the transcriptomic response of human alveolar epithelial cells exposed to S. aurantiacum conidia paving a way for better understanding of the mechanism of the infection process.

LanguageEnglish
Article number5035
Number of pages10
JournalScientific Reports
Volume9
DOIs
Publication statusPublished - 22 Mar 2019

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Scedosporium
Fungal Spores
Epithelial Cells
Lung
Infection
Alveolar Epithelial Cells
NF-kappa B
Extracellular Space
Cystic Fibrosis
Transcriptional Activation
Microscopy
Immune System
Fungi
Cytokines
Genes
A549 Cells

Bibliographical note

Copyright the Author(s) 2019. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this

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title = "Interactions of an emerging fungal pathogen Scedosporium aurantiacum with human lung epithelial cells",
abstract = "Scedosporium fungi are found in various natural and host-associated environments, including the lungs of cystic fibrosis patients. However, their role in infection development remains underexplored. Here the attachment of conidia of a virulent S. aurantiacum strain WM 06.482 onto the human lung epithelial A549 cells in vitro was visualized using microscopy to examine the initial steps of infection. We showed that 75–80{\%} of fungal conidia were bound to the A549 cells within four hours of co-incubation, and started to produce germ tubes. The germinating conidia seemed to invade the cells through the intercellular space, no intracellular uptake of fungal conidia by the airway epithelial cells after conidial attachment. Transcriptomic analysis of the A549 cells revealed that the up-regulated genes were mainly associated with cell repair and inflammatory processes indicating a protective response against S. aurantiacum infection. Network analysis of the differentially expressed genes showed activation of the innate immune system (NF-kB pathway) leading to the release of pro-inflammatory cytokines. We believe this is the first report showing the transcriptomic response of human alveolar epithelial cells exposed to S. aurantiacum conidia paving a way for better understanding of the mechanism of the infection process.",
author = "Jashanpreet Kaur and Liisa Kautto and Anahit Penesyan and Wieland Meyer and Elbourne, {Liam D. H.} and Paulsen, {Ian T.} and Helena Nevalainen",
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Interactions of an emerging fungal pathogen Scedosporium aurantiacum with human lung epithelial cells. / Kaur, Jashanpreet; Kautto, Liisa; Penesyan, Anahit; Meyer, Wieland; Elbourne, Liam D. H.; Paulsen, Ian T.; Nevalainen, Helena.

In: Scientific Reports, Vol. 9, 5035, 22.03.2019.

Research output: Contribution to journalArticleResearchpeer-review

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