Interleukin-12 and interferon-γ are not detectable in the cerebrospinal fluid of patients with amyotrophic lateral sclerosis

Lara S. Ford; Dominic B. Rowe

doi:10.1080/14660820410019125

Interleukin-12 and interferon-γ are not detectable in the cerebrospinal fluid of patients with amyotrophic lateral sclerosis

Lara S. Ford, Dominic B. Rowe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The cause of sporadic amyotrophic lateral sclerosis (SALS) is unknown. We investigated the immune-mediated inflammatory hypothesis of SALS by assaying interleukin-12 (IL-12), interleukin-6 (IL-6) and interferon-gamma (IFN-γ) in the cerebrospinal fluid (CSF) of patients with SALS. These cytokines were measured in the CSF from patients with SALS (n=11), patients with immune-mediated inflammatory central nervous system or nerve root disorders (n=12), and patients with other neurological diseases (n=15) by high sensitivity sandwich enzyme linked immunosorbent assay (ELISA). All samples were below the assay detection limits of O.5 pg/ml for IL-12 and 8 pg/ml for IFN-γ. There was no difference between the groups in the mean concentration of IL-6. There is no evidence in cerebrospinal fluid for induction of a T_H1 immune response in SALS.

Original language	English
Pages (from-to)	118-120
Number of pages	3
Journal	Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders
Volume	5
Issue number	2
DOIs	https://doi.org/10.1080/14660820410019125
Publication status	Published - Jun 2004
Externally published	Yes

Keywords

Amyotrophic lateral sclerosis
Cerebrospinal fluid
Cytokines
Enzyme-linked immunosorbent assay
Motor neuron disease

Access to Document

10.1080/14660820410019125

Cite this

@article{10f341e482a14ef49c35a2ba1396fd2f,

title = "Interleukin-12 and interferon-γ are not detectable in the cerebrospinal fluid of patients with amyotrophic lateral sclerosis",

abstract = "The cause of sporadic amyotrophic lateral sclerosis (SALS) is unknown. We investigated the immune-mediated inflammatory hypothesis of SALS by assaying interleukin-12 (IL-12), interleukin-6 (IL-6) and interferon-gamma (IFN-γ) in the cerebrospinal fluid (CSF) of patients with SALS. These cytokines were measured in the CSF from patients with SALS (n=11), patients with immune-mediated inflammatory central nervous system or nerve root disorders (n=12), and patients with other neurological diseases (n=15) by high sensitivity sandwich enzyme linked immunosorbent assay (ELISA). All samples were below the assay detection limits of O.5 pg/ml for IL-12 and 8 pg/ml for IFN-γ. There was no difference between the groups in the mean concentration of IL-6. There is no evidence in cerebrospinal fluid for induction of a TH1 immune response in SALS.",

keywords = "Amyotrophic lateral sclerosis, Cerebrospinal fluid, Cytokines, Enzyme-linked immunosorbent assay, Motor neuron disease",

author = "Ford, {Lara S.} and Rowe, {Dominic B.}",

year = "2004",

month = jun,

doi = "10.1080/14660820410019125",

language = "English",

volume = "5",

pages = "118--120",

journal = "Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders",

issn = "1466-0822",

publisher = "Taylor & Francis",

number = "2",

}

TY - JOUR

T1 - Interleukin-12 and interferon-γ are not detectable in the cerebrospinal fluid of patients with amyotrophic lateral sclerosis

AU - Ford, Lara S.

AU - Rowe, Dominic B.

PY - 2004/6

Y1 - 2004/6

N2 - The cause of sporadic amyotrophic lateral sclerosis (SALS) is unknown. We investigated the immune-mediated inflammatory hypothesis of SALS by assaying interleukin-12 (IL-12), interleukin-6 (IL-6) and interferon-gamma (IFN-γ) in the cerebrospinal fluid (CSF) of patients with SALS. These cytokines were measured in the CSF from patients with SALS (n=11), patients with immune-mediated inflammatory central nervous system or nerve root disorders (n=12), and patients with other neurological diseases (n=15) by high sensitivity sandwich enzyme linked immunosorbent assay (ELISA). All samples were below the assay detection limits of O.5 pg/ml for IL-12 and 8 pg/ml for IFN-γ. There was no difference between the groups in the mean concentration of IL-6. There is no evidence in cerebrospinal fluid for induction of a TH1 immune response in SALS.

AB - The cause of sporadic amyotrophic lateral sclerosis (SALS) is unknown. We investigated the immune-mediated inflammatory hypothesis of SALS by assaying interleukin-12 (IL-12), interleukin-6 (IL-6) and interferon-gamma (IFN-γ) in the cerebrospinal fluid (CSF) of patients with SALS. These cytokines were measured in the CSF from patients with SALS (n=11), patients with immune-mediated inflammatory central nervous system or nerve root disorders (n=12), and patients with other neurological diseases (n=15) by high sensitivity sandwich enzyme linked immunosorbent assay (ELISA). All samples were below the assay detection limits of O.5 pg/ml for IL-12 and 8 pg/ml for IFN-γ. There was no difference between the groups in the mean concentration of IL-6. There is no evidence in cerebrospinal fluid for induction of a TH1 immune response in SALS.

KW - Amyotrophic lateral sclerosis

KW - Cerebrospinal fluid

KW - Cytokines

KW - Enzyme-linked immunosorbent assay

KW - Motor neuron disease

UR - http://www.scopus.com/inward/record.url?scp=3843130871&partnerID=8YFLogxK

U2 - 10.1080/14660820410019125

DO - 10.1080/14660820410019125

M3 - Article

C2 - 15204013

AN - SCOPUS:3843130871

SN - 1466-0822

VL - 5

SP - 118

EP - 120

JO - Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders

JF - Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders

IS - 2

ER -

Interleukin-12 and interferon-γ are not detectable in the cerebrospinal fluid of patients with amyotrophic lateral sclerosis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this