International validation of a natural-killer-cell–based model to predict recurrence-free survival in hepatocellular carcinoma

Miho Akabane, Jun Kawashima, Selamawit Woldesenbet, François Cauchy, Federico Aucejo, Irinel Popescu, Minoru Kitago, Guillaume Martel, Francesca Ratti, Luca Aldrighetti, George A. Poultsides, Yuki Imaoka, Andrea Ruzzenente, Itaru Endo, Ana Gleisner, Hugo P. Marques, Vincent Lam, Tom Hugh, Nazim Bhimani, Feng ShenTimothy M. Pawlik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Models estimating recurrence-free survival (RFS) after hepatectomy for hepatocellular carcinoma (HCC) rely on clinical variables and often overlook tumor immunobiology. The Liver Immune Status Index (LISI), derived from BMI, albumin, and Fibrosis-4 (FIB-4), reflects liver-resident natural killer (NK) cell activity. We validated the HISCO-HCC score, combining LISI, tumor burden score (TBS), and alpha-fetoprotein (AFP), using an international cohort. Methods: Patients undergoing curative-intent hepatectomy for HCC (2000–2023) were identified from an international database (median follow-up: 38.9 [14.9–67.5] months). RFS was the primary endpoint. LISI's predictive performance was compared with other liver-related indices. The original HISCO-HCC (oHISCO-HCC) was recalibrated via multivariable Cox regression in a training cohort (80 %) stratified by region, yielding a modified score (mHISCO-HCC). Validation was conducted in the testing cohort (20 %). Results: Among 1213 patients, LISI had the highest AUCs among liver-related indices for 1-/2-year RFS (0.60/0.60) and 1-/5-year OS (0.64/0.60). The formula: mHISCO-HCC = 0.49 × TBS + 0.41 × log(AFP) + 0.13 × LISI. In testing, mHISCO-HCC outperformed oHISCO-HCC and mHALT-HCC for 12-/36-/60-month RFS (AUCs: 0.73/0.71/0.66) with the lowest AIC. It also had the highest OS AUCs and stratified RFS and OS (p < 0.001). Conclusions: The mHISCO-HCC score, integrating tumor morphology, biology, and NK cell-based immunity, improves prediction of recurrence and survival. It may aid postoperative stratification.

Original languageEnglish
Pages (from-to)1259-1269
Number of pages11
JournalHPB
Volume27
Issue number10
Early online date3 Jul 2025
DOIs
Publication statusPublished - Oct 2025
Externally publishedYes

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