Intervertebral disc recovery after dynamic or static loading in vitro: Is there a role for the endplate?

A. J. van der Veen*, J. H. van Dieën, A. Nadort, B. Stam, T. H. Smit

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

In vivo studies on disc mechanics show loss of fluid from the intervertebral disc (IVD) during loading and full recovery during rest. Previous work indicated that in vitro recovery is hampered after static loading. The aim of the present study was to investigate the role of the endplate after dynamic and static loading on mechanical recovery in vitro. Lumbar spines (caprine) were obtained from the local slaughterhouse and stored frozen. Twenty-four intervertebral discs were thawed and subjected to a compression test in a saline bath (37 °C). The discs were pre-loaded at 20 N for 15 min. Three 15-min loading cycles (static: 2.0 MPa or dynamic: average load 2.0 MPa at 0.5 Hz) were applied, each followed by a 30-min period of unloading (20 N). After this protocol, the endplates of half of the discs were blocked with silicone paste and the long-term recovery protocol was applied; the discs were subjected to a single loading cycle (15 min of static or dynamic loading) followed by 10 h of unloading at 20 N. All specimens showed a net loss of height and a gain in stiffness during the first part of the test. Eventually, height and stiffness were restored during a long-term recovery test. The difference in recovery between blocked and free endplates was marginal. If fluid flow plays a role during recovery in vitro, the role of the endplate appears to be limited. Our findings show no influence of loading type on recovery in vitro.

Original languageEnglish
Pages (from-to)2230-2235
Number of pages6
JournalJournal of Biomechanics
Volume40
Issue number10
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Fluid flow
  • Intervertebral disc
  • Mechanical testing
  • Spine

Fingerprint Dive into the research topics of 'Intervertebral disc recovery after dynamic or static loading in vitro: Is there a role for the endplate?'. Together they form a unique fingerprint.

Cite this