Into the wild: dissemination of antibiotic resistance determinants via a species recovery program

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Management strategies associated with captive breeding of endangered species can establish opportunities for transfer of pathogens and genetic elements between human and animal microbiomes. The class 1 integron is a mobile genetic element associated with clinical antibiotic resistance in gram-negative bacteria. We examined the gut microbiota of endangered brush-tail rock wallabies Petrogale penicillata to determine if they carried class 1 integrons. No integrons were detected in 65 animals from five wild populations. In contrast, class 1 integrons were detected in 48% of fecal samples from captive wallabies. The integrons contained diverse cassette arrays that encoded resistance to streptomycin, spectinomycin, and trimethoprim. Evidence suggested that captive wallabies had acquired typical class 1 integrons on a number of independent occasions, and had done so in the absence of strong selection afforded by antibiotic therapy. Sufficient numbers of bacteria containing diverse class 1 integrons must have been present in the general environment occupied by the wallabies to account for this acquisition. The captive wallabies have now been released, in an attempt to bolster wild populations of the species. Consequently, they can potentially spread resistance integrons into wild wallabies and into new environments. This finding highlights the potential for genes and pathogens from human sources to be acquired during captive breeding and to be unwittingly spread to other populations.

LanguageEnglish
Article numbere63017
Pages1-5
Number of pages5
JournalPLoS ONE
Volume8
Issue number5
DOIs
Publication statusPublished - 22 May 2013

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Integrons
Macropodidae
Pathogens
Microbial Drug Resistance
antibiotic resistance
Bacteria
Animals
Interspersed Repetitive Sequences
Spectinomycin
Anti-Bacterial Agents
Recovery
Trimethoprim
Streptomycin
Brushes
Genes
Rocks
Petrogale
Breeding
spectinomycin
Diplopoda

Bibliographical note

Copyright the Author(s) 2013. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Cite this

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title = "Into the wild: dissemination of antibiotic resistance determinants via a species recovery program",
abstract = "Management strategies associated with captive breeding of endangered species can establish opportunities for transfer of pathogens and genetic elements between human and animal microbiomes. The class 1 integron is a mobile genetic element associated with clinical antibiotic resistance in gram-negative bacteria. We examined the gut microbiota of endangered brush-tail rock wallabies Petrogale penicillata to determine if they carried class 1 integrons. No integrons were detected in 65 animals from five wild populations. In contrast, class 1 integrons were detected in 48{\%} of fecal samples from captive wallabies. The integrons contained diverse cassette arrays that encoded resistance to streptomycin, spectinomycin, and trimethoprim. Evidence suggested that captive wallabies had acquired typical class 1 integrons on a number of independent occasions, and had done so in the absence of strong selection afforded by antibiotic therapy. Sufficient numbers of bacteria containing diverse class 1 integrons must have been present in the general environment occupied by the wallabies to account for this acquisition. The captive wallabies have now been released, in an attempt to bolster wild populations of the species. Consequently, they can potentially spread resistance integrons into wild wallabies and into new environments. This finding highlights the potential for genes and pathogens from human sources to be acquired during captive breeding and to be unwittingly spread to other populations.",
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Into the wild : dissemination of antibiotic resistance determinants via a species recovery program. / Power, Michelle L.; Emery, Samantha; Gillings, Michael R.

In: PLoS ONE, Vol. 8, No. 5, e63017, 22.05.2013, p. 1-5.

Research output: Contribution to journalArticleResearchpeer-review

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