Intra- and extraerythrocyte pH at 37°C and during long term storage at 4°C: 31P NMR measurements and an electrochemical model of the system

J. E. Raftos; B. E. Chapman; P. W. Kuchel; V. A. Lovric; I. M. Stewart

Intra- and extraerythrocyte pH at 37°C and during long term storage at 4°C: ³¹P NMR measurements and an electrochemical model of the system

J. E. Raftos^*, B. E. Chapman, P. W. Kuchel, V. A. Lovric, I. M. Stewart

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A ³¹P NMR method based on pH dependent variation of the chemical-shift-difference between the resonances of orthophosphate and methylphosphonate was used to measure simultaneously intracellular pH (pH_i) and extracellular pH (pHc) during long term storage of erythrocytes; pH was determined at both 4°C and 37°C. An equation describing the equilibrium distribution of membrane-permeant ions was derived by consideration of the electrochemical and osmotic constraints in the RBC suspension. Calculations using the model-equation and the measured pH_i yielded the Donnan ratio and therefore pH_o; the relationship between experimentally determined pH_i and pH_o values was accurately predicted by the model. Sensitivity analysis of the model-equation revealed that the observed increase in transmembrane pH gradient during storage is principally due to the alteration of the total net charge of intracellular (poly)-anions.

Original language	English
Pages (from-to)	251-268
Number of pages	18
Journal	Haematologia
Volume	19
Issue number	4
Publication status	Published - 1986

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title = "Intra- and extraerythrocyte pH at 37°C and during long term storage at 4°C: 31P NMR measurements and an electrochemical model of the system",

abstract = "A 31P NMR method based on pH dependent variation of the chemical-shift-difference between the resonances of orthophosphate and methylphosphonate was used to measure simultaneously intracellular pH (pHi) and extracellular pH (pHc) during long term storage of erythrocytes; pH was determined at both 4°C and 37°C. An equation describing the equilibrium distribution of membrane-permeant ions was derived by consideration of the electrochemical and osmotic constraints in the RBC suspension. Calculations using the model-equation and the measured pHi yielded the Donnan ratio and therefore pHo; the relationship between experimentally determined pHi and pHo values was accurately predicted by the model. Sensitivity analysis of the model-equation revealed that the observed increase in transmembrane pH gradient during storage is principally due to the alteration of the total net charge of intracellular (poly)-anions.",

author = "Raftos, {J. E.} and Chapman, {B. E.} and Kuchel, {P. W.} and Lovric, {V. A.} and Stewart, {I. M.}",

year = "1986",

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T1 - Intra- and extraerythrocyte pH at 37°C and during long term storage at 4°C

T2 - 31P NMR measurements and an electrochemical model of the system

AU - Raftos, J. E.

AU - Chapman, B. E.

AU - Kuchel, P. W.

AU - Lovric, V. A.

AU - Stewart, I. M.

PY - 1986

Y1 - 1986

N2 - A 31P NMR method based on pH dependent variation of the chemical-shift-difference between the resonances of orthophosphate and methylphosphonate was used to measure simultaneously intracellular pH (pHi) and extracellular pH (pHc) during long term storage of erythrocytes; pH was determined at both 4°C and 37°C. An equation describing the equilibrium distribution of membrane-permeant ions was derived by consideration of the electrochemical and osmotic constraints in the RBC suspension. Calculations using the model-equation and the measured pHi yielded the Donnan ratio and therefore pHo; the relationship between experimentally determined pHi and pHo values was accurately predicted by the model. Sensitivity analysis of the model-equation revealed that the observed increase in transmembrane pH gradient during storage is principally due to the alteration of the total net charge of intracellular (poly)-anions.

AB - A 31P NMR method based on pH dependent variation of the chemical-shift-difference between the resonances of orthophosphate and methylphosphonate was used to measure simultaneously intracellular pH (pHi) and extracellular pH (pHc) during long term storage of erythrocytes; pH was determined at both 4°C and 37°C. An equation describing the equilibrium distribution of membrane-permeant ions was derived by consideration of the electrochemical and osmotic constraints in the RBC suspension. Calculations using the model-equation and the measured pHi yielded the Donnan ratio and therefore pHo; the relationship between experimentally determined pHi and pHo values was accurately predicted by the model. Sensitivity analysis of the model-equation revealed that the observed increase in transmembrane pH gradient during storage is principally due to the alteration of the total net charge of intracellular (poly)-anions.

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SN - 0017-6559

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JF - Haematologia

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ER -

Intra- and extraerythrocyte pH at 37°C and during long term storage at 4°C: ³¹P NMR measurements and an electrochemical model of the system

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