Intramolecular DNA coiling mediated by metallo-supramolecular cylinders

differential binding of P and M helical enantiomers

Isabelle Meistermann, Virtudes Moreno, Maria J. Prieto, Erlend Moldrheim, Einar Sletten, Syma Khalid, P. Mark Rodger, Jemma C. Peberdy, Christian J. Isaac, Alison Rodger, Michael J. Hannon*

*Corresponding author for this work

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 107 M-1 and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.

Original languageEnglish
Pages (from-to)5069-5074
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number8
DOIs
Publication statusPublished - 16 Apr 2002
Externally publishedYes

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