Prior work has shown that activation of metabotropic glutamate receptors can induce burst firing and a form of NMDA receptor independent long term potentiation in lateral septal slice preparations. To study this phenomenon in vivo we used the expression of immediate early gene products as markers for increased neuronal activity following intraseptal injection of the metabotropic agonist 1S,3R-ACPD. Intraseptal injection of 1S,3R-ACPD induced the expression of Fos-like, Jun B-like and Krox24-like immunoreactivity in lateral septal neurons in a dose-dependent fashion. Immediate early gene product expression peaked at 4 to 6 h post-injection and then declined to baseline. Immediate early gene expression was diminished by co-injection of L-AP3 and was not elicited by intraseptal injection of L-AP4, cysteine sulfinic acid or DHPG. Immediate early gene expression was not diminished by chronic lithium treatment but was diminished by chronic treatment with the phospholipase A2 inhibitor quinacrine. Co-injection of the phospholipase A2 inhibitor NDGA partially suppressed the induction of immediate early gene expression. Metabotropic glutamate receptors regulate lateral septal neuron excitability in vivo and some of their effects may be mediated by activation of phospholipase A2. Alternatively, arachidonic acid may play a permissive role in the effects of metabotropic glutamate receptors on lateral septal neurons.
- Excitatory amino acid
- Metabotropic glutamate