Intron-spanning primers for the amplification of the nuclear ANT gene in decapod crustaceans

Peter R. Teske, Luciano B. Beheregaray

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)


    We describe polymerase chain reaction primers that amplify the low-copy nuclear adenine nucleotide transporter gene in decapod crustaceans. These were tested on 35 species from 14 decapod families, and a single polymerase chain reaction product amplified in 32 species. Of 49 sequences generated, only two did not contain an intron, and the longest intron identified was more than 834 nucleotides in length. The amplified fragment is likely to be useful at various taxonomic levels. While the intron is suitable for phylogeographical/population genetic studies and to identify cryptic speciation, the second exon region is sufficiently long to provide signal at both the phylogeographical and phylogenetic levels.

    Original languageEnglish
    Pages (from-to)774-776
    Number of pages3
    JournalMolecular Ecology Resources
    Issue number3
    Publication statusPublished - May 2009


    • ATP/ADP translocase
    • Cryptic species
    • EPIC primers
    • Nuclear DNA
    • Phylogenetics
    • Phylogeography


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