TY - JOUR
T1 - Isoelectric focusing in a silica nanofluidic channel
T2 - effects of electromigration and electroosmosis
AU - Hsu, Wei Lun
AU - Inglis, David W.
AU - Startsev, Michael A.
AU - Goldys, Ewa M.
AU - Davidson, Malcolm R.
AU - Harvie, Dalton J. E.
PY - 2014/9/2
Y1 - 2014/9/2
N2 - Isoelectric focusing of proteins in a silica nanofluidic channel filled with citric acid and disodium phosphate buffers is investigated via numerical simulation. Ions in the channel migrate in response to (i) the electric field acting on their charge and (ii) the bulk electroosmotic flow (which is directed toward the cathode). Proteins are focused near the low pH (anode) end when the electromigration effect is more significant and closer to the high pH (cathode) end when the electroosmotic effect dominates. We simulate the focusing behavior of Dylight labeled streptavidin (Dyl-Strep) proteins in the channel, using a relationship between the protein's charge and pH measured in a previous experiment. Protein focusing results compare well to previous experimental measurements. The effect of some key parameters, such as applied voltage, isoelectric point (pI), bulk pH, and bulk conductivity, on the protein trapping behavior in a nanofluidic channel is examined.
AB - Isoelectric focusing of proteins in a silica nanofluidic channel filled with citric acid and disodium phosphate buffers is investigated via numerical simulation. Ions in the channel migrate in response to (i) the electric field acting on their charge and (ii) the bulk electroosmotic flow (which is directed toward the cathode). Proteins are focused near the low pH (anode) end when the electromigration effect is more significant and closer to the high pH (cathode) end when the electroosmotic effect dominates. We simulate the focusing behavior of Dylight labeled streptavidin (Dyl-Strep) proteins in the channel, using a relationship between the protein's charge and pH measured in a previous experiment. Protein focusing results compare well to previous experimental measurements. The effect of some key parameters, such as applied voltage, isoelectric point (pI), bulk pH, and bulk conductivity, on the protein trapping behavior in a nanofluidic channel is examined.
UR - http://www.scopus.com/inward/record.url?scp=84906860975&partnerID=8YFLogxK
U2 - 10.1021/ac501875u
DO - 10.1021/ac501875u
M3 - Article
C2 - 25098739
AN - SCOPUS:84906860975
SN - 0003-2700
VL - 86
SP - 8711
EP - 8718
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 17
ER -