Isolation and characterisation of microsatellite loci in the bush stone-curlew (burhinus grallarius), a declining Australian bird

Robert A B Mason*, Catherine Price, Walter E. Boles, Karen Anne Gray, Edwina Rickard, Mark D B Eldridge, Rebecca N. Johnson

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The bush stone-curlew (Burhinus grallarius Latham), a ground-nesting nocturnal bird, is endangered in southern Australia due to habitat modification and introduced predators. To provide tools for conservation, ecological and behavioural studies, we isolated variable microsatellite repeat sequences and designed primers for PCR amplification in this species. Primer pairs were developed and levels of diversity were assessed for eight microsatellite loci, including one locus linked to the gene encoding Microtubule-Associated Protein 2, a protein important for behavioural imprinting in birds, and one sex-linked locus. Isolated loci contained allelic diversity of between 5 and 17 alleles.

Original languageEnglish
Pages (from-to)421-423
Number of pages3
JournalAustralian Journal of Zoology
Volume61
Issue number6
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Burhinidae
  • conservation genetics
  • hemizygous
  • hybridisation capture and enrichment
  • microsatellite
  • Microtubule-Associated Protein 2 (MAP2)
  • non-isotopic screening

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