Isolation and development of a molecular sex marker for Bassiana duperreyi, a lizard with XX/XY sex chromosomes and temperature-induced sex reversal

Alexander E. Quinn, Rajkumar S. Radder, Stephen D. Sarre, Arthur Georges, Tariq Ezaz, Richard Shine

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Sex determination in the endemic Australian lizard Bassiana duperreyi (Scincidae) is influenced by sex chromosomes and incubation temperature, challenging the traditional dichotomy in reptilian sex determination. Analysis of those interactions requires sex chromosome markers to identify temperature-induced sex reversal. Here, we report the isolation of Y chromosome DNA sequence from B. duperreyi using amplified fragment length polymorphism PCR, the conversion of that sequence to a single-locus assay, and its combination with a single-copy nuclear gene (C-mos) to form a duplex PCR test for chromosomal sex. The accuracy of the assay was tested on an independent panel of individuals with known phenotypic sex. When used on offspring from field nests, our test identified the likely occurrence of a low rate of natural sex reversal in this species. This work represents the first report of Y chromosome sequence from a reptile and one of the few reptile sex tests.

Original languageEnglish
Pages (from-to)665-672
Number of pages8
JournalMolecular Genetics and Genomics
Volume281
Issue number6
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

Keywords

  • GSD
  • TSD
  • AFLP
  • reptile
  • Y chromosome

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