Isolation and identification of phenolic compounds in Citrus sinensis

Prasan Swatsitang; Greg Tucker; Kevin Robards; Daniel Jardine

doi:10.1016/S0003-2670(00)00937-5

Isolation and identification of phenolic compounds in Citrus sinensis

Prasan Swatsitang, Greg Tucker, Kevin Robards, Daniel Jardine

Department of Molecular Sciences

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

Various methods were compared for the recovery of phenolic compounds from sweet orange. The need for careful consideration of sample preparation was demonstrated. In the case of cinnamic acids, hydroxide-treatment was essential to liberate the bound acids. Ferulic acid was the predominant phenol in alkali-treated extracts. Positive and negative ion electrospray ionisation mass spectra were systematically investigated for a range of phenols. Detection limits were significantly lower in negative ion than in positive ion mode. However, positive ion mode provided additional structural information for many of the compounds.

Original language	English
Pages (from-to)	231-240
Number of pages	10
Journal	Analytica Chimica Acta
Volume	417
Issue number	2
DOIs	https://doi.org/10.1016/S0003-2670(00)00937-5
Publication status	Published - 2000

Keywords

Citrus
HPLC
Mass spectrometry
Phenols
Sample preparation

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AB - Various methods were compared for the recovery of phenolic compounds from sweet orange. The need for careful consideration of sample preparation was demonstrated. In the case of cinnamic acids, hydroxide-treatment was essential to liberate the bound acids. Ferulic acid was the predominant phenol in alkali-treated extracts. Positive and negative ion electrospray ionisation mass spectra were systematically investigated for a range of phenols. Detection limits were significantly lower in negative ion than in positive ion mode. However, positive ion mode provided additional structural information for many of the compounds.

KW - Citrus

KW - HPLC

KW - Mass spectrometry

KW - Phenols

KW - Sample preparation

U2 - 10.1016/S0003-2670(00)00937-5

DO - 10.1016/S0003-2670(00)00937-5

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