Isolation and maintenance of nonadherent quiescent human monocytes for studies of adhesion and migration

Paul G. Bannon, Roger T. Dean, Joan Dawes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A method is described for the isolation and culture of large numbers of nonadherent quiescent human peripheral blood monocytes for adhesion/migration studies. Approximately 1.0×108 cells/white cell concentrate with > 95% viability are isolated by countercurrent centrifugal elutriation. These cells are initially activated by the isolation procedure, but after a minimum culture time post isolation of 48 hours and a rest period after radiola-belling of at least 3 hours quiescent cells are routinely obtained, with low basal levels of adhesion and a rapid, reproducible superoxide response to activation with phorbol myristate acetate.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalMethods in Cell Science
Volume17
Issue number1
DOIs
Publication statusPublished - Mar 1995
Externally publishedYes

Keywords

  • Activation
  • Adhesion
  • Isolation
  • Migration
  • Monocyte
  • Superoxide

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