Isolation and maintenance of nonadherent quiescent human monocytes for studies of adhesion and migration

Paul G. Bannon; Roger T. Dean; Joan Dawes

doi:10.1007/BF00981886

Isolation and maintenance of nonadherent quiescent human monocytes for studies of adhesion and migration

Paul G. Bannon, Roger T. Dean, Joan Dawes^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A method is described for the isolation and culture of large numbers of nonadherent quiescent human peripheral blood monocytes for adhesion/migration studies. Approximately 1.0×10⁸ cells/white cell concentrate with > 95% viability are isolated by countercurrent centrifugal elutriation. These cells are initially activated by the isolation procedure, but after a minimum culture time post isolation of 48 hours and a rest period after radiola-belling of at least 3 hours quiescent cells are routinely obtained, with low basal levels of adhesion and a rapid, reproducible superoxide response to activation with phorbol myristate acetate.

Original language	English
Pages (from-to)	53-59
Number of pages	7
Journal	Methods in Cell Science
Volume	17
Issue number	1
DOIs	https://doi.org/10.1007/BF00981886
Publication status	Published - Mar 1995
Externally published	Yes

Keywords

Activation
Adhesion
Isolation
Migration
Monocyte
Superoxide

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10.1007/BF00981886

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title = "Isolation and maintenance of nonadherent quiescent human monocytes for studies of adhesion and migration",

abstract = "A method is described for the isolation and culture of large numbers of nonadherent quiescent human peripheral blood monocytes for adhesion/migration studies. Approximately 1.0×108 cells/white cell concentrate with > 95% viability are isolated by countercurrent centrifugal elutriation. These cells are initially activated by the isolation procedure, but after a minimum culture time post isolation of 48 hours and a rest period after radiola-belling of at least 3 hours quiescent cells are routinely obtained, with low basal levels of adhesion and a rapid, reproducible superoxide response to activation with phorbol myristate acetate.",

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author = "Bannon, {Paul G.} and Dean, {Roger T.} and Joan Dawes",

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T1 - Isolation and maintenance of nonadherent quiescent human monocytes for studies of adhesion and migration

AU - Bannon, Paul G.

AU - Dean, Roger T.

AU - Dawes, Joan

PY - 1995/3

Y1 - 1995/3

N2 - A method is described for the isolation and culture of large numbers of nonadherent quiescent human peripheral blood monocytes for adhesion/migration studies. Approximately 1.0×108 cells/white cell concentrate with > 95% viability are isolated by countercurrent centrifugal elutriation. These cells are initially activated by the isolation procedure, but after a minimum culture time post isolation of 48 hours and a rest period after radiola-belling of at least 3 hours quiescent cells are routinely obtained, with low basal levels of adhesion and a rapid, reproducible superoxide response to activation with phorbol myristate acetate.

AB - A method is described for the isolation and culture of large numbers of nonadherent quiescent human peripheral blood monocytes for adhesion/migration studies. Approximately 1.0×108 cells/white cell concentrate with > 95% viability are isolated by countercurrent centrifugal elutriation. These cells are initially activated by the isolation procedure, but after a minimum culture time post isolation of 48 hours and a rest period after radiola-belling of at least 3 hours quiescent cells are routinely obtained, with low basal levels of adhesion and a rapid, reproducible superoxide response to activation with phorbol myristate acetate.

KW - Activation

KW - Adhesion

KW - Isolation

KW - Migration

KW - Monocyte

KW - Superoxide

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SP - 53

EP - 59

JO - Methods in Cell Science

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