Abstract
A method is described for the isolation and culture of large numbers of nonadherent quiescent human peripheral blood monocytes for adhesion/migration studies. Approximately 1.0×108 cells/white cell concentrate with > 95% viability are isolated by countercurrent centrifugal elutriation. These cells are initially activated by the isolation procedure, but after a minimum culture time post isolation of 48 hours and a rest period after radiola-belling of at least 3 hours quiescent cells are routinely obtained, with low basal levels of adhesion and a rapid, reproducible superoxide response to activation with phorbol myristate acetate.
Original language | English |
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Pages (from-to) | 53-59 |
Number of pages | 7 |
Journal | Methods in Cell Science |
Volume | 17 |
Issue number | 1 |
DOIs | |
Publication status | Published - Mar 1995 |
Externally published | Yes |
Keywords
- Activation
- Adhesion
- Isolation
- Migration
- Monocyte
- Superoxide