Isolation and purification of plant secondary metabolites using column-chromatographic technique

Vivek K. Bajpai*, Rajib Majumder, Jae Gyu Park

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)
144 Downloads (Pure)


Chromatographic techniques have significant role in natural products chemistry as well as contribute dramatically in the discovery of novel and innovative compounds of pharmaceutical and biomedical importance. This study focused on step-by-step visual demonstration of fractionation and isolation of biologically active plant secondary metabolites using column-chromatographic techniques. Isolation of bioactive compounds using column-chromatographic involves: a) Preparation of sample; b) Packing of column; c) Pouring of sample into the column; d) Elution of fractions; and e) Analysis of each fractions using thin layer chromatography. However, depending on nature of research, compounds can be further purified using high performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) spectral analyses.

Original languageEnglish
Pages (from-to)844-848
Number of pages5
JournalBangladesh Journal of Pharmacology
Issue number4
Publication statusPublished - 2016
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.


  • Column chromatography
  • Metabolite
  • Plant


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