Isolation, characterization, and cross-amplisfication of microsatellite markers for the petunia integrifolia (Solanaceae) complex

Raquel A. Kriedt; Aline M C Ramos-Fregonezi; Luciano B. Beheregaray; Sandro L. Bonatto; Loreta B. Freitas

doi:10.3732/ajb.1100178

Isolation, characterization, and cross-amplisfication of microsatellite markers for the petunia integrifolia (Solanaceae) complex

Raquel A. Kriedt, Aline M C Ramos-Fregonezi, Luciano B. Beheregaray, Sandro L. Bonatto, Loreta B. Freitas

Department of Biological Sciences

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

Premise of the study: Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida. Methods and Results: We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex. Conclusions: The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.

Original language	English
Pages (from-to)	e277–e279
Number of pages	3
Journal	American Journal of Botany
Volume	98
Issue number	10
DOIs	https://doi.org/10.3732/ajb.1100178
Publication status	Published - Oct 2011

Keywords

Congeneric transferability
Microsatellite
Petunia integrifolia complex

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10.3732/ajb.1100178

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abstract = "Premise of the study: Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida. Methods and Results: We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex. Conclusions: The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.",

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AU - Kriedt, Raquel A.

AU - Ramos-Fregonezi, Aline M C

AU - Beheregaray, Luciano B.

AU - Bonatto, Sandro L.

AU - Freitas, Loreta B.

PY - 2011/10

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AB - Premise of the study: Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida. Methods and Results: We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex. Conclusions: The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.

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