Isolation, characterization, and cross-amplisfication of microsatellite markers for the petunia integrifolia (Solanaceae) complex

Raquel A. Kriedt, Aline M C Ramos-Fregonezi, Luciano B. Beheregaray, Sandro L. Bonatto, Loreta B. Freitas

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Abstract

Premise of the study: Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida. Methods and Results: We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex. Conclusions: The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.

Original languageEnglish
Pages (from-to)e277–e279
Number of pages3
JournalAmerican Journal of Botany
Volume98
Issue number10
DOIs
Publication statusPublished - Oct 2011

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Keywords

  • Congeneric transferability
  • Microsatellite
  • Petunia integrifolia complex

Cite this

Kriedt, R. A., Ramos-Fregonezi, A. M. C., Beheregaray, L. B., Bonatto, S. L., & Freitas, L. B. (2011). Isolation, characterization, and cross-amplisfication of microsatellite markers for the petunia integrifolia (Solanaceae) complex. American Journal of Botany, 98(10), e277–e279. https://doi.org/10.3732/ajb.1100178