Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera:Muscidae)

Rachael Y. Dudaniec, Michael G. Gardner*, Sonia Kleindorfer

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.

Original languageEnglish
Pages (from-to)142-144
Number of pages3
JournalMolecular Ecology Resources
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 2008
Externally publishedYes

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