TY - JOUR
T1 - Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera:Muscidae)
AU - Dudaniec, Rachael Y.
AU - Gardner, Michael G.
AU - Kleindorfer, Sonia
PY - 2008/1
Y1 - 2008/1
N2 - An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.
AB - An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.
UR - http://www.scopus.com/inward/record.url?scp=36849030765&partnerID=8YFLogxK
U2 - 10.1111/j.1471-8286.2007.01900.x
DO - 10.1111/j.1471-8286.2007.01900.x
M3 - Article
C2 - 21585739
AN - SCOPUS:36849030765
SN - 1755-098X
VL - 8
SP - 142
EP - 144
JO - Molecular Ecology Resources
JF - Molecular Ecology Resources
IS - 1
ER -