Isolation, concentration and pre-fractionation of DNA adducts, glycans and glycoproteins using novel chemically modified carbon/graphite (CPAK) sample preparation devices

Alexander V. Lazarev*, Nicolle H. Packer, Mikhail B. Bogdanov, Wayne R. Matson

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

Concentration, isolation and pre-fractionation of DNA adducts, glycans and glycoproteins was analyzed by using chemically modified carbn/graphite (CPAK) sample preparation devices. It was observed that higher surface hydroxylation provides unique binding affinity for selected classes of compounds including nucleotide derivatives, glycans and glycopeptides in the CAPK devices. Tightly controlled porosity, rigid column structure and chemical resistance of the devices allowed a rapid clean-up and regeneration for multiple use. These devices can be used in a wide variety of applications such as desalting and high-throughput in-gel enzymatic treatment of glycoproteins.

Original languageEnglish
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Place of PublicationSanta Fe, NM
PublisherAmerican Society for Mass Spectrometry (ASMS)
Pages603-604
Number of pages2
Publication statusPublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2 Jun 20026 Jun 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period2/06/026/06/02

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  • Cite this

    Lazarev, A. V., Packer, N. H., Bogdanov, M. B., & Matson, W. R. (2002). Isolation, concentration and pre-fractionation of DNA adducts, glycans and glycoproteins using novel chemically modified carbon/graphite (CPAK) sample preparation devices. In Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 603-604). Santa Fe, NM: American Society for Mass Spectrometry (ASMS).