TY - JOUR
T1 - Isolation of circulating tumor cells from seminal fluid of patients with prostate cancer using inertial microfluidics
AU - Rzhevskiy, Alexey S.
AU - Kapitannikova, Alina Y.
AU - Vasilescu, Steven A.
AU - Karashaeva, Tamilla A.
AU - Razavi Bazaz, Sajad
AU - Taratkin, Mark S.
AU - Enikeev, Dmitry V.
AU - Lekarev, Vladimir Y.
AU - Shpot, Evgeniy V.
AU - Butnaru, Denis V.
AU - Deyev, Sergey M.
AU - Thiery, Jean Paul
AU - Zvyagin, Andrei V.
AU - Ebrahimi Warkiani, Majid
N1 - Copyright © 2022 by the authors. Licensee MDPI, Basel, Switzerland. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2022/7
Y1 - 2022/7
N2 - Prostate cancer (PCa) diagnosis is primarily based on prostate-specific antigen (PSA) testing and prostate tissue biopsies. However, PSA testing has relatively low specificity, while tissue biopsies are highly invasive and have relatively low sensitivity at early stages of PCa. As an alternative, we developed a technique of liquid biopsy, based on isolation of circulating tumor cells (CTCs) from seminal fluid (SF). The recovery of PCa cells from SF was demonstrated using PCa cell lines, achieving an efficiency and throughput as high as 89% (±3.8%) and 1.7 mL min−1, respectively, while 99% (±0.7%) of sperm cells were disposed of. The introduced approach was further tested in a clinical setting by collecting and processing SF samples of PCa patients. The yield of isolated CTCs measured as high as 613 cells per SF sample in comparison with that of 6 cells from SF of healthy donors, holding significant promise for PCa diagnosis. The correlation analysis of the isolated CTC numbers with the standard prognostic parameters such as Gleason score and PSA serum level showed correlation coefficient values at 0.40 and 0.73, respectively. Taken together, our results show promise in the developed liquid biopsy technique to augment the existing diagnosis and prognosis of PCa.
AB - Prostate cancer (PCa) diagnosis is primarily based on prostate-specific antigen (PSA) testing and prostate tissue biopsies. However, PSA testing has relatively low specificity, while tissue biopsies are highly invasive and have relatively low sensitivity at early stages of PCa. As an alternative, we developed a technique of liquid biopsy, based on isolation of circulating tumor cells (CTCs) from seminal fluid (SF). The recovery of PCa cells from SF was demonstrated using PCa cell lines, achieving an efficiency and throughput as high as 89% (±3.8%) and 1.7 mL min−1, respectively, while 99% (±0.7%) of sperm cells were disposed of. The introduced approach was further tested in a clinical setting by collecting and processing SF samples of PCa patients. The yield of isolated CTCs measured as high as 613 cells per SF sample in comparison with that of 6 cells from SF of healthy donors, holding significant promise for PCa diagnosis. The correlation analysis of the isolated CTC numbers with the standard prognostic parameters such as Gleason score and PSA serum level showed correlation coefficient values at 0.40 and 0.73, respectively. Taken together, our results show promise in the developed liquid biopsy technique to augment the existing diagnosis and prognosis of PCa.
KW - circulating tumor cells
KW - liquid biopsy
KW - microfluidics
KW - prostate cancer
KW - seminal fluid
UR - http://www.scopus.com/inward/record.url?scp=85136366387&partnerID=8YFLogxK
U2 - 10.3390/cancers14143364
DO - 10.3390/cancers14143364
M3 - Article
C2 - 35884424
AN - SCOPUS:85136366387
SN - 2072-6694
VL - 14
SP - 1
EP - 14
JO - Cancers
JF - Cancers
IS - 14
M1 - 3364
ER -