Ketimine reductase/CRYM catalyzes reductive alkylamination of α-keto acids, confirming its function as an imine reductase

André Hallen, Arthur J L Cooper, Jason R. Smith, Joanne F. Jamie, Peter Karuso

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Recently, crystalized mouse ketimine reductase/CRYM complexed with NADPH was found to have pyruvate bound in its active site. We demonstrate that the enzyme binds α-keto acids, such as pyruvate, in solution, and catalyzes the formation of N-alkyl-amino acids from alkylamines and α-keto acids (via reduction of imine intermediates), but at concentrations of these compounds not expected to be encountered in vivo. These findings confirm that, mechanistically, ketimine reductase/CRYM acts as a classical imine reductase and may explain the finding of bound pyruvate in the crystallized protein.

LanguageEnglish
Pages2457-2461
Number of pages5
JournalAmino Acids
Volume47
Issue number11
DOIs
Publication statusPublished - 1 Nov 2015

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Keto Acids
Imines
Pyruvic Acid
Oxidoreductases
NADP
Catalytic Domain
Amino Acids
Enzymes
ketimine reductase
Proteins

Cite this

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Ketimine reductase/CRYM catalyzes reductive alkylamination of α-keto acids, confirming its function as an imine reductase. / Hallen, André; Cooper, Arthur J L; Smith, Jason R.; Jamie, Joanne F.; Karuso, Peter.

In: Amino Acids, Vol. 47, No. 11, 01.11.2015, p. 2457-2461.

Research output: Contribution to journalArticleResearchpeer-review

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