Abstract
Recently, crystalized mouse ketimine reductase/CRYM complexed with NADPH was found to have pyruvate bound in its active site. We demonstrate that the enzyme binds α-keto acids, such as pyruvate, in solution, and catalyzes the formation of N-alkyl-amino acids from alkylamines and α-keto acids (via reduction of imine intermediates), but at concentrations of these compounds not expected to be encountered in vivo. These findings confirm that, mechanistically, ketimine reductase/CRYM acts as a classical imine reductase and may explain the finding of bound pyruvate in the crystallized protein.
| Language | English |
|---|---|
| Pages | 2457-2461 |
| Number of pages | 5 |
| Journal | Amino Acids |
| Volume | 47 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 1 Nov 2015 |
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Ketimine reductase/CRYM catalyzes reductive alkylamination of α-keto acids, confirming its function as an imine reductase. / Hallen, André; Cooper, Arthur J L; Smith, Jason R.; Jamie, Joanne F.; Karuso, Peter.
In: Amino Acids, Vol. 47, No. 11, 01.11.2015, p. 2457-2461.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Ketimine reductase/CRYM catalyzes reductive alkylamination of α-keto acids, confirming its function as an imine reductase
AU - Hallen, André
AU - Cooper, Arthur J L
AU - Smith, Jason R.
AU - Jamie, Joanne F.
AU - Karuso, Peter
PY - 2015/11/1
Y1 - 2015/11/1
N2 - Recently, crystalized mouse ketimine reductase/CRYM complexed with NADPH was found to have pyruvate bound in its active site. We demonstrate that the enzyme binds α-keto acids, such as pyruvate, in solution, and catalyzes the formation of N-alkyl-amino acids from alkylamines and α-keto acids (via reduction of imine intermediates), but at concentrations of these compounds not expected to be encountered in vivo. These findings confirm that, mechanistically, ketimine reductase/CRYM acts as a classical imine reductase and may explain the finding of bound pyruvate in the crystallized protein.
AB - Recently, crystalized mouse ketimine reductase/CRYM complexed with NADPH was found to have pyruvate bound in its active site. We demonstrate that the enzyme binds α-keto acids, such as pyruvate, in solution, and catalyzes the formation of N-alkyl-amino acids from alkylamines and α-keto acids (via reduction of imine intermediates), but at concentrations of these compounds not expected to be encountered in vivo. These findings confirm that, mechanistically, ketimine reductase/CRYM acts as a classical imine reductase and may explain the finding of bound pyruvate in the crystallized protein.
UR - http://www.scopus.com/inward/record.url?scp=84944735345&partnerID=8YFLogxK
U2 - 10.1007/s00726-015-2044-8
DO - 10.1007/s00726-015-2044-8
M3 - Article
VL - 47
SP - 2457
EP - 2461
JO - Amino Acids
T2 - Amino Acids
JF - Amino Acids
SN - 0939-4451
IS - 11
ER -