Abstract
The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the minimal SNAP-25 binding domain in KIF5B to residues 874-894. Overexpression of a fragment of KIF5B (residues 594-910) resulted in significant colocalization with SNAP-25 with resulting blockage of the trafficking of SNAP-25 to the periphery of cells. This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events.
Original language | English |
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Pages (from-to) | 388-393 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 391 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Jan 2010 |
Externally published | Yes |
Keywords
- Binding Sites
- Cell Membrane
- HeLa Cells
- Humans
- Kinesin
- Protein Structure, Tertiary
- Protein Transport
- Synaptic Vesicles
- Synaptosomal-Associated Protein 25
- Journal Article
- Research Support, Non-U.S. Gov't