Kinesin-1 plays a role in transport of SNAP-25 to the plasma membrane

April M Morton; Anthony L. Cunningham; Russell J Diefenbach

doi:10.1016/j.bbrc.2009.11.068

Kinesin-1 plays a role in transport of SNAP-25 to the plasma membrane

April M Morton, Anthony L. Cunningham, Russell J Diefenbach

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the minimal SNAP-25 binding domain in KIF5B to residues 874-894. Overexpression of a fragment of KIF5B (residues 594-910) resulted in significant colocalization with SNAP-25 with resulting blockage of the trafficking of SNAP-25 to the periphery of cells. This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events.

Original language	English
Pages (from-to)	388-393
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	391
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.11.068
Publication status	Published - 1 Jan 2010
Externally published	Yes

Keywords

Binding Sites
Cell Membrane
HeLa Cells
Humans
Kinesin
Protein Structure, Tertiary
Protein Transport
Synaptic Vesicles
Synaptosomal-Associated Protein 25
Journal Article
Research Support, Non-U.S. Gov't

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title = "Kinesin-1 plays a role in transport of SNAP-25 to the plasma membrane",

abstract = "The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the minimal SNAP-25 binding domain in KIF5B to residues 874-894. Overexpression of a fragment of KIF5B (residues 594-910) resulted in significant colocalization with SNAP-25 with resulting blockage of the trafficking of SNAP-25 to the periphery of cells. This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events.",

keywords = "Binding Sites, Cell Membrane, HeLa Cells, Humans, Kinesin, Protein Structure, Tertiary, Protein Transport, Synaptic Vesicles, Synaptosomal-Associated Protein 25, Journal Article, Research Support, Non-U.S. Gov't",

author = "Morton, {April M} and Cunningham, {Anthony L.} and Diefenbach, {Russell J}",

year = "2010",

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AU - Morton, April M

AU - Cunningham, Anthony L.

AU - Diefenbach, Russell J

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N2 - The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the minimal SNAP-25 binding domain in KIF5B to residues 874-894. Overexpression of a fragment of KIF5B (residues 594-910) resulted in significant colocalization with SNAP-25 with resulting blockage of the trafficking of SNAP-25 to the periphery of cells. This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events.

AB - The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the minimal SNAP-25 binding domain in KIF5B to residues 874-894. Overexpression of a fragment of KIF5B (residues 594-910) resulted in significant colocalization with SNAP-25 with resulting blockage of the trafficking of SNAP-25 to the periphery of cells. This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events.

KW - Binding Sites

KW - Cell Membrane

KW - HeLa Cells

KW - Humans

KW - Kinesin

KW - Protein Structure, Tertiary

KW - Protein Transport

KW - Synaptic Vesicles

KW - Synaptosomal-Associated Protein 25

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.bbrc.2009.11.068

DO - 10.1016/j.bbrc.2009.11.068

M3 - Article

VL - 391

SP - 388

EP - 393

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

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